组织蛋白酶V对乳腺癌MCF-7细胞增殖抑制作用的研究  

Inhibition of Cathepsin V on proliferation of breast cancer cell line MCF-7

作　　者：周慧[1] 韩翰[2] 侯昕彤 刘春阳 孙玮璐 周伟强[4] ZHOU Hui;HAN Han;HOU Xin-tong;LIU Chun-yang;SUN Wei-lu;ZHOU Wei-qiang(Dept of Histology and Embryology, Shenyang Medical College, Shenyang Liaoning 110034, China;Dept of Biochemistry, Shenyang Medical College, Shenyang Liaoning 110034, China;School of Basic Medical Science, Shenyang Medical College, Shenyang Liaoning 110034, China;Dept of Pathogen Biology/Key Laboratory of Environmental Pollution and Microecology of Liaoaing Province, Shenyang Medical College, Shenyang Liaoning 110034, China)

机构地区：[1]沈阳医学院组织学与胚胎学教研室,辽宁沈阳110034 [2]沈阳医学院生物化学教研室,辽宁沈阳110034 [3]沈阳医学院基础医学院,辽宁沈阳110034 [4]沈阳医学院病原生物学教研室/辽宁省环境污染与微生态重点实验室,辽宁沈阳110034

出　　处：《毒理学杂志》2018年第3期216-220,共5页Journal of Toxicology

基　　金：国家自然科学基金(81172509);沈阳医学院科技基金(20172035);沈阳医学院大学生科研立项(20179018);辽宁省级大学生创新创业训练计划项目(201710164000024)

摘　　要：目的探讨组织蛋白酶V(CTSV)对乳腺癌MCF-7细胞增殖的抑制作用。方法以人乳腺癌MCF-7细胞为研究对象,分为正常对照组和SAHA(0.5、1、2、5、10、20和50μmol/L)组。MUSE自动细胞分析仪检测辛二酰苯胺异羟肟酸(SAHA)对MCF-7细胞活力的影响,实时荧光定量PCR技术检测MCF-7细胞中微管相关蛋白1轻链3 A(LC3A)和CTSV mRNA表达变化的情况,Western blot检测CTSV蛋白的表达水平。结果 MUSE自动细胞分析仪结果显示,与正常对照组比较,经过SAHA处理后MCF-7细胞活力明显下降(P<0.01)。实时荧光定量PCR结果表明,LC3A和CTSV mRNA水平在SAHA的作用下增加(P<0.01)。Western blot结果发现,SAHA可以促进CTSV蛋白表达增加(P<0.05)。结论 CTSV在SAHA诱导的乳腺癌细胞增殖抑制过程中起到重要的调控作用。Objective To investigate the inhibitory effect of Cathepsin V on proliferation of breast cancer cell MCF-7. Method Human breast cell line MCF-7 was evaluated by the cell viability and cell apoptosis by muse cell analyzer. The mRNA levels of LC3 A and CTSV in MCF-7 cells were detected by real time PCR. Western blot was performed to detect the level of CTSV protein. Results After the treatment of SAHA,the ability of cell proliferation and cell viability were depressed（ P 0. 01）. Real-time PCR array result showed that SAHA increased the expression of LC3 A and CTSV（ P 0. 01）. Western blot showed that the level of CTSV protein increased（ P 0. 05）.Conclusion CTSV plays an important role in the inhibition of proliferation induced by SAHA.

关 键 词：乳腺癌 CTSV SAHA 

分 类 号：R329.24[医药卫生—人体解剖和组织胚胎学]