人参皂苷Rb1通过抑制炎症反应及氧化应激改善老年小鼠内皮功能  

Ginsenoside Rb1 Improves Endothelial Function via Inhibiton of Inflammation and oxidative stress in Aged Mice

作　　者：宋志明 余舒杰[2] 巩贵宏[1] 杨建涛 张海波 钱孝贤 SONG Zhi-ming;YU Shu-jie;GONG Gui-hong(Department of Cardiology, The First Affiliated Hospital, Henan University, Kaifeng feng 475001, China;Department of Cardiology, The Third Affiliated Hospital , San Yat-sen University , Guangzhou 510630,China;Instiute on Aging and Disease , Henan University , Kai fen g 475001 ,China;Institute for Integrated Traditional Chinese and Western Medicine, Sun Yat-sen University ,Guangzhou 510630, China)

机构地区：[1]河南大学第一附属医院心内科,河南开封475001 [2]中山大学附属第三医院心内科,广东广州510630 [3]河南大学衰老与疾病研究所,河南开封475001 [4]中山大学中西医结合研究所,广东广州510630

出　　处：《中国实验诊断学》2018年第6期1066-1070,共5页Chinese Journal of Laboratory Diagnosis

基　　金：国家自然科学基金(81370447);河南省科技发展计划(182123100559);广东省科技计划(2016A050502014);开封市科技发展计划(1708003);河南大学科研基金(B2016108;2016YBZR002)

摘　　要：目的探讨人参皂苷Rb1对老年鼠主动脉内皮功能的影响及其可能机制。方法 10只雌性C57BL/6小鼠为年轻对照组(3月);将30只雌性老年鼠(22月)随机分为老年对照组,老年Rb1低剂量组(10mg/kg Rb1)和老年Rb1高剂量组(20mg/kg Rb1)。分别给予相应处理6周,验证人参皂苷Rb1对老年鼠主动脉内皮炎症指标及氧化应激的作用。结果与年轻鼠相比,老年鼠体内炎症指标单核细胞趋化蛋白-1(MCP-1)、纤溶酶原激活物抑制因子1(PAI-1)、细胞黏附分子-1(ICAM-1)和丙二醛(MDA)表达均显著增加;而超氧化物歧化酶1(SOD1)、内皮型一氧化氮合酶(eNOS)和一氧化氮(NO)表达明显减少。给予不同剂量Rb1后,前述指标表达均得到一定程度的逆转。结论人参皂苷Rb1通过抑制老年鼠体内炎症反应和氧化应激,从而发挥改善主动脉内皮功能的作用。Objective To explore the effect of ginsenoside Rb1（Rb1）on the endothelial function in aged mice and the possibly involved mechanisms.Methods 40 female mice were divided to young control group,aged control group,aged L-Rb1 group and aged H-Rb1 group.The mice were intraperitoneally injected with Rb1 or normal saline respectively for 6 weeks.And then several parameters were detected to demonstrate the effect of Rb1 on inflammation and oxidative stress.Results Compared to young control mice,there were more expression of MCP-1,PAI-1,ICAM-1 and MDA,while less expression of SOD1,eNOS and NO.Rb1 treatment was able to reverse the expression profile of parameters mentioned above.Conclusion Ginsenoside Rb1 Improves Endothelial Function via Inhibiton of Inflammation and oxidative stress in Aged Mice.

关 键 词：人参皂苷RB1 炎症 氧化应激 一氧化氮 

分 类 号：R285[医药卫生—中药学]