棘白霉素B脱酰基酶高产菌株的选育及转化条件优化  

Screening of high echinocandin B deacylase-producing strain and optimization of biotransformation conditions

作　　者：郄丽萍[1] 王小连 马婕[1] 钱思宇[1] 代明伟[1] 张炜[1] Qie Li-ping;Wang Xiao-lian;Ma Jie;Qian Si-yu;Dai Ming-wei;Zhang Wei(NCPC New Drug Research and Development Co.,Ltd., National Engineerin;Research Center of Microbial Medicine, Hebei Industry Microbial Metabolic Engineering ＆ Technology Research Center, Shijiazhuang 05001)

机构地区：[1]华北制药集团新药研究开发有限责任公司微生物药物国家工程研究中心河北省工业微生物代谢工程技术研究中心,石家庄050015

出　　处：《中国抗生素杂志》2018年第6期696-700,共5页Chinese Journal of Antibiotics

基　　金：河北省科技条件建设项目(No.169676404G);河北省引进留学人员资助项目(No.CL201617)

摘　　要：目的为提高棘白霉素B脱酰基酶产生菌的转化率。方法采用常压室温等离子体诱变复合链霉素抗性筛选转化高产菌株;对新菌株转化棘白霉素B的底物溶剂、转化温度和转化时间等条件进行优化。结果获得稳定突变株ZH-6-78比出发菌的转化率提高了26.7%;优化后转化条件为:转化温度35℃,转化时间24h。结论高产菌种在优化的转化条件下,转化率达到90%,比出发菌原工艺提高了50.0%,为工业化生产奠定基础。Objective To enhance the efficiency of the conversion of the echinocandin B deacylase-producing strain. Methods The echinocandin B deacylase-producing strain was treated by ARTP, followed by resistance selection with streptomycin; Biotransformation conditions including the solvent of precursor, temperature, and time were optimized to increase the transformation ratio of echinocandin B. Results A stable mutant strain ZH-6-78 was obtained and its conversion efficiency was 26.7% higher than that of the original strain. The optimum conversion conditions were determined as follows： the conversion temperature was 35℃, and the conversion time was 24h. Conclusion The biotransformation percentage of echinocandin B for the new strain was 90% under the optimized conversion conditions. It was 50% higher than that of original strain under the initial conversion conditions, which laid the foundation for the industrial production.

关 键 词：棘白霉素B 脱酰基酶 转化 优化 

分 类 号：R978.1[医药卫生—药品]