A-40926混合物中B_0组分的分离纯化  

Separation and purification of component B_0 in antibiotic A-40926

作　　者：宋盼[1] 郭月玲[1] 王崔岩[1] 彭亮[1] 倪会敏[1] 仲伟潭[1] Song Pan;Guo Yue-ling;Wang Cui-yan;Peng Liang;Ni Hui-min;Zhong Wei-tan(National Engineering Research Center of Microbial Medicine, Hebei Industry Microbial Metabolic Engineering ＆ Technology Research Center, New Drug Research ＆ Development Company ofNCPC, Shijiazhuang 050015)

机构地区：[1]微生物药物国家工程研究中心河北省工业微生物代谢工程技术研究中心华北制药集团新药研究开发有限责任公司,石家庄050015

出　　处：《中国抗生素杂志》2018年第6期701-704,共4页Chinese Journal of Antibiotics

摘　　要：目的开发一种高纯度A-40926混合物中B_0组分(以下简称A-40926 B_0)的分离纯化工艺。方法采用中压色谱系统,以聚合物微球为载体,A-40926 B_0的收率为指标,筛选聚合物微球的类型,优化洗脱条件。结果型号为Uni PS30-300的微球为最佳层析介质,上样量为10mg(每毫升填料),洗脱剂依次为体积分数30%,45%的乙醇溶液,流速为1.5BV/h(BV:层析柱体积);所得产品纯度大于95%,层析总收率大于75%。结论此种高纯度A-40926 B_0的分离纯化方法简单易行,收率稳定,适于工业化生产。Objective To develop the separation and purification process of B0 component in the A-40926 mixture （here in after referred to as A-40926 B0）. Methods Using medium pressure chromatography system, the polymer microspheres were used as carriers and the yield of A-40926 B0 as indexes. The types of polymer microspheres were screened and the elution conditions were optimized. Results The type of UniPS30-300 polymer particles were selected as the best chromatographic media. The optimal conditions were as follows： The sample loading amount was 10mg （per lmL filler）, and 30% and 45% ethanol solution was used as the elution solution at a flow rate of 1.SBV/h （BV： column volume）. The purity of the product was higher than 95% and the yield was above 75%. Conclusion The established purification process ofA-40926 B0 was simple to operate and the yield was high and feasible. Thus the orocess was suitable for industrialized production.

关 键 词：A-40926 B0 聚合物微球 分离纯化 

分 类 号：R987.1[医药卫生—药品]