96点阵琼脂稀释法与微量肉汤稀释法药敏试验结果的对比  

Comparison of the results of 96 dot agar dilution method and broth microdilution method

作　　者：胡明[1,2] 李璐璐[1,2] 赵敏 骆延波[1,2] 张印 齐静[1,2] 张庆[1,2] 刘玉庆[1,2] Hu Ming;Li Lu-lu;Zhao Min;Luo Yan-bo;Zhang Yin;Qi Jing;Zhang Qing;Liu Yu-qing(Institute of Animal Husbandry and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan 250101;Shandong Provincial Key Laboratory of Animal Disease Control and Breeding, Ji＇nan 250101)

机构地区：[1]山东省农业科学院畜牧兽医研究所,济南250101 [2]山东省畜禽疫病防治与繁育重点实验室,济南250101

出　　处：《中国抗生素杂志》2018年第6期729-733,共5页Chinese Journal of Antibiotics

基　　金：国家自然科学基金(No.81271886和No.31672609);山东省农业重大应用技术创新项目(No.SDAIT-11-09和No.CXGC2016A10);山东省自然科学培养基金(No.ZR2014CP021)

摘　　要：目的为了验证96点阵琼脂稀释法进行药敏试验的可靠性和有效性,与国际通用的微量肉汤稀释法进行对比。方法用微量肉汤稀释法和96点阵琼脂稀释法分别对质控菌株E.coli ATCC25922、临床分离E.coli进行4种不同药物(头孢噻呋、庆大霉素、氟苯尼考和多西环素)的药敏试验,并经过多次重复和对比。结果这两种检测方法所测定的菌株的最低抑菌浓度(minimal inhibitory concentration,MIC)完全相同者占30%~50%,相差1个梯度占80%,3个梯度以内的吻合率高达96%以上,96点阵琼脂稀释法具有很好的重复性和稳定性。结论 96点阵琼脂稀释法与微量肉汤稀释法两种方法测定的MIC没有显著差异,可共用一个质控标准。96点阵琼脂稀释法的标准化和规范化,有利于高通量药敏检测的进行,推动建立完善的抗药性监测技术体系。Objective To confirm the reliability and the effectiveness of 96 dot agar dilution method in antimicrobial susceptibility test, the results were contrasted to those of the international standard of broth microdilution method. Methods The control strain E. coli ATCC25922 and clinical isolates E. coli were used to be tested the minimal inhibitory concentration （MIC） by the broth dilution method and 96 agar dilution method respectively for four different antimicrobial drugs （ceftiofur, gentamicin, florfenicol and doxycycline）. The result data were repeated and compared. Results The MICs determined by two methods were identical accounting for 30%-50%, with a difference of one gradients accounting for 80%, and within three gradients, the coincidence rate was as high as 96%. The 96 dot matrix agar dilution method had good repeatability and stability. Conclusion There was no significant difference between the two methods of the broth microdilution method and the 96 broth dilution method on MIC detected, and the same quality control standard could be shared. The standardization and normalization of the 96 dot agar dilution method was conducive to the development of high throughput antimicrobial sensitivity detection, and to promote the establishment of a perfect drug resistance monitoring system.

关 键 词：药敏试验 96点阵琼脂稀释法 微量肉汤稀释法 

分 类 号：Q939.99[生物学—微生物学]