白及丝氨酸羟甲基转移酶(SHMT)基因的序列分析  

Bioinformatic Analysis of Serine Hydroxymethyltransferase(SHMT) Gene in Bletilla striata

作　　者：沈访 李林[2] 潘胤池 张社波 马激 徐德林[2] SHEN Fang;LI Lin;PAN Yin-Chi;ZHANG She-Bo;MA Ji;XU De-Lin(Affiliated Hospital of Zunyi Medical University,Zunyi 563000;Department of Cell Biology,Zunyi Medical University,Zunyi 563099)

机构地区：[1]遵义医学院附属医院,遵义563000 [2]遵义医学院细胞生物学教研室,遵义563099

出　　处：《植物研究》2018年第4期543-550,共8页Bulletin of Botanical Research

基　　金：国家自然科学基金项目(31560079;31560087);贵州省科学技术基金项目(黔科合LH字[2014]7549号);贵州省中药现代化科技产业研究开发专项项目(黔科合ZY字[2013]3002号);遵义市2014年度"15851"人才工程项目(201424);遵义医学院精英人才工程项目~~

摘　　要：丝氨酸羟甲基转移酶(SHMT)是植物细胞光合作用和一碳代谢的关键酶之一,研究SHMT基因的序列信息对揭示其蛋白结构与功能具有重要指导意义。从白及转录组数据库中分离得到SHMT基因(NCBI登录号:MG544187),运用生物信息学软件对该基因进行序列分析。结果显示:该基因长度为1 953 bp,编码的蛋白质长度为471aa、分子量为51.861 06 k D、理论等电点为7.17,SHMT二级结构主要由无规则卷曲结构和α螺旋结构组成,核苷酸和氨基酸序列与铁皮石斛SHMT相似性最高,达93%,结构域分析发现该蛋白具有高度保守结构域,三级结构预测为四聚体结构,跨膜区及信号肽分析发现该蛋白无跨膜区及信号肽,亚细胞定位分析发现该蛋白主要位于细胞质、叶绿体亚细胞器位置。本分析结果为白及SHMT的应用研究提供了基础数据,也为植物SHMT基因的分子研究提供了理论依据及基础资料。Serine hydroxymethyl transferase （SHMT） is a key enzyme in the processes of one-carbon metabolism and photosynthesis. Exploring its sequence information through bioinformatics analyzing could comprehensively peep at the protein structure and functions. We picked out the SHMT sequence （NCBI accession ID： MG544187） from the transcriptome of Bletilla striata（Thunb. ） Rchb. f. , and then applied a series of tools to explore the biological information of this gene. We detected that this gene has 1 953 bp in length and coding 471 amino acid residues, which sharing the highest similarity with the SHMT gene of Dendrobium catenatum as 93%. The protein had a predicted molecular weight of 51. 861 06 kD and theoretical isoelectric point of 7.17. According to the protein＇ s secondary structure, we revealed it shared several highly conserved domains with other species＇ SHMT genes. And its tertiary was a homo-tetramer structure without transmembrane components and signal peptide but contained several phosphorylation sites. Through the subcellular localization analysis, this protein was mainly located in cytoplasm and Chloroplast. This analysis lays a solid foundation for future studies on SHMT＇s function confirming, mechanism revelation and application for genetically improving in B.striata. This study also provides some basic data for studies on SHMT genes of other species.

关 键 词：白及 丝氨酸羟甲基转移酶 序列分析 结构预测 

分 类 号：Q949.95[生物学—植物学]