短瓣金莲花种子无菌萌发与幼苗快繁技术初探  

Tissue Culture and Rapid Propagation of Trollius ledebouri

作　　者：周芳[1] 徐剑秋 安娟艳 蒋洪毛 王珍熹 张天星 李开隆[1] ZHOU Fang;XU Jian-Qiu;AN Juan-Yan;ZHANG Tian-Xing;JIANG Hong-Mao;WANG Zhen-Xi;LI Kai-Long(State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040)

机构地区：[1]东北林业大学林木遗传育种国家重点实验室,哈尔滨150040

出　　处：《植物研究》2018年第4期619-624,共6页Bulletin of Botanical Research

基　　金：大学生创新训练计划项目(201620225015)~~

摘　　要：以野生短瓣金莲花种子为材料,探讨不同处理方式对种子发芽的影响,并比较不同培养基类型及激素组合对短瓣金莲花根茎部位形成的愈伤组织、不定芽增殖及生长的影响,建立无菌播种及组培快繁体系,为短瓣金莲花快繁和规模化生产提供技术支撑。结果表明:(1)用600 mg·L^(-1)GA_3溶液浸泡48 h后并用0.1%HgCl_2溶液灭菌20 min的种子发芽效果最好;(2)适宜于增殖的培养基配方为MS+0.5 mg·L^(-1)6-BA+0.1 mg·L^(-1)NAA;(3)适宜于生根的培养基配方为不添加激素的MS。The seeds of Trollius ledebouri Reichb. were used by omerent concentrauot~ a ~ to promote germination, and their buds were inoculated on different types of medium with different hormone. A rapid propagation system by tissue culture was established and become basis for large-scale production of T. ledebouri. The results showed that ： （ 1 ） the seeds of T. ledebouri Reichb was efficiently sterilized by 0.1% HgCl2 for 20 min after soaking in 600 mg· L- 1 GA3 solution for 48 h and the germination was high ; （2） the suitable medium for multiplication of T. ledebouri was MS ＋ 0.5 mg · L- 1 6-BA ＋ 0.1 mg · L- 1 NAA; （ 3 ） the suitable rooting medium was MS without any hormone,

关 键 词：短瓣金莲花 组织培养 快速扩繁 

分 类 号：Q949.95[生物学—植物学]