Crim1在肥大心肌组织中的表达及AT1R对其表达的调控作用  

The expression of Crim1 in hypertrophic myocardial tissue and the regulating effect of AT1R on the process

作　　者：何炯红[1] 杨龙[1] 夏桂玲 杨君[1,3] 唐倩[1,4] 邓娜 杨英[1] 扶泽南 黄勇淇[1] HE Jionghong;YANG Long;XIA Guiling;YANG Jun;TANGQian;DENG Na;YANG Ying;FU Ze＇nan;HUANG Yongqi(Department of Cardiology;Department of Geratology, Guizhou Provincial People＇s Hospital, Guiyang, 550002, China;Department of Cardiology, the First Affiliated Hospital of Guiyang U- niversity of Chinese Medicine;Department of Cardiology, the Affiliated Hospital of Guizhou Medical University)

机构地区：[1]贵州省人民医院贵州医科大学附属人民医院心内科,贵阳550002 [2]贵州省人民医院干医科 [3]贵阳中医学院第一附属医院心内科 [4]贵州医科大学附属医院心内科

出　　处：《临床心血管病杂志》2018年第6期609-612,共4页Journal of Clinical Cardiology

基　　金：国家自然科学基金(No:81260040)

摘　　要：目的:探讨半胱氨酸丰富跨膜成骨蛋白调控因子1(Crim1)在肥大心肌组织中的表达情况以及血管紧张素受体1型(AT1R)对其蛋白表达的调控作用。方法:将220~250g SD清洁级雄鼠随机分为4组:腹部假手术+等体积0.9%氯化钠溶液灌胃(对照组),腹主动脉缩窄术+等体积0.9%氯化钠溶液灌胃(AAC组),腹主动脉缩窄术+替米沙坦灌胃(3.57mg·kg^(-1)·d^(-1))(AAC+T组),腹部假手术+替米沙坦灌胃(3.57mg·kg^(-1)·d^(-1))(T组)。超声心动图测量各组大鼠左心室各室壁厚度、左心室内径,并计算左心室质量(校正值);HE染色检测各组大鼠心室肌细胞横截面积,Western blot、Crim组织化学检测各组大鼠心室肌细胞Crim1蛋白的表达。结果:腹主动脉缩窄术诱导的大鼠肥大心室肌中的Crim1蛋白表达下调,而替米沙坦可抑制此下调过程。结论:肥大心肌组织中Crim1蛋白表达下调,AT1R信号通路可能介导了肥大心肌组织中Crim1蛋白表达的调控。Objective：To investigate the expression of cysteine-rich motor neuron 1（Crim1）protein in hypertrophic myocardial tissues and the effect of angiotensin receptor 1 type（AT1R）on Crim1 protein expression.Method：220-250 g Sprague-Dawley（SD）rats were randomly divided into 4 groups：control group（sham operation）,AAC group（abdominal aortic constriction）,AAC＋T group（abdominal aorta constriction plus Telmisartan,3.57 mg·kg^-1·d^-1）,Telmisartan group（sham operation plus Telmisartan,3.57 mg·kg^-1·d^-1）.The left ventricular（LV）wall thickness,LV internal dimension and corrected LV mass were measured by echocardiography.The cross-sectional area of cardiomyocytes was monitored by HE staining.Crim1 protein expression was detected by Western blotting and immunohistochemical staining.Result：The expression of Crim1 protein was significantly reduced in AAC-induced hypertrophic myocardial tissues in SD rats,while Telmisartan prevented this effect.Conclusion：The expression of Crim1 protein is down-regulated in hypertrophic myocardial tissues,which might be regulated by AT1R.

关 键 词：心肌肥大 半胱氨酸丰富跨膜成骨蛋白调控因子1 血管紧张素受体1型 腹主动脉缩窄 

分 类 号：R542.2[医药卫生—心血管疾病]