橡胶树乳管细胞中与HbMADS4互作蛋白的筛选  

Screening of Proteins Interacting with HbMADS4 from the Laticifer Cells of Hevea brasiliensis

作　　者：李辉亮 陈月异[1] 郭冬 王颖[2] 朱家红[2] 彭世清[1] Li Huiliang;Chen Yueyi;Guo Dong;Wang Ying;Zhu Jiahong;Peng Shiqing(Danzhou Investigation ＆ Experiment Station of Tropical Cmps, Ministry of Agriculture, Hainan Key Laboratory of Cultivation ＆ Physiology for Tropical Crops, State Key Laboratory Breeding Base Built by Provincial Ministry, Key Laboratory of Rubber Biology and Genetic Resource Utilization, Ministry of Agriculture, Danzhou, 571737;Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101)

机构地区：[1]农业部橡胶树生物学与遗传资源利用重点实验室、省部共建国家重点实验室培育基地、海南省热带作物栽培生理学重点实验室、农业部儋州热带作物科学观测实验站,儋州571737 [2]中国热带农业科学院热带生物技术研究所、农业部热带作物生物学与遗传资源利用重点实验室,海口571101

出　　处：《基因组学与应用生物学》2018年第6期2498-2504,共7页Genomics and Applied Biology

基　　金：农业部儋州热带作物科学观测实验站开放课题基金项目(No.RRI-KLOF201504)资助

摘　　要：MADS-box转录因子在植物的生长发育、花器官的形成、信号传导、参与次生代谢物合成等方面发挥重要的作用。在前期研究中我们获得了一个在橡胶树自根幼态无性系和供体胶乳中差异表达的MADS-box转录因子基因HbMADS4,初步发现HbMADS4负调控小橡胶粒子蛋白基因（HbSRPP）的表达。为深入研究HbMADS4的生物学功能,以HbMADS4为诱饵蛋白,利用酵母双杂交从橡胶树胶乳cDNA文库中筛选与其相互作用的蛋白。通过阳性克隆的筛选、复筛、回转验证和生物信息学分析,初步获得了10个与诱饵蛋白相互作用的靶蛋白。对这些靶蛋白的功能分析,将为进一步研究HbMADS4在橡胶树乳管细胞中的生物学功能提供重要信息。MADS-box transcription factors play important roles in plant growth and development, floral organ formation, signal transduction, and participation in secondary metabolite synthesis. In the previous study, we obtained a differentially expressed MADS-box transcription factor gene, HbMADS4, in self-rooting immature clones and donor latex of Hevea brasiliensis. It was preliminarily found that HbMADS4 negatively regulated the expression of HbSRPP. In order to further study the biological function of HbMADS4, yeast two-hybrid was performed using HbMADS4 as bait protein to screen the interacting proteins from the Hevea brasiliensis cDNA library. A total of 10 target proteins interacting with HbMADS4 were obtained by screening, re-screening, rotary validation and bioinformatics analysis of positive clones. The functional analysis of these target proteins might provide important information for the study on the biological function of HbMADS4 in the laticifer cells of Hevea brasiliensis.

关 键 词：橡胶树 HbMADS4 酵母双杂交 蛋白质相互作用 

分 类 号：S794.1[农业科学—林木遗传育种]