增强型超分子荧光探针对拉帕替尼的识别及其在细胞成像中的应用  

A Turn-On Supramolecular Fluorescent Probe for Sensing Lapatinib and Its Application in Living Cell Imaging

作　　者：王成会 唐青 席芸芸 杨梅[2] 李涛[2] 黄英 陶朱[2] Wang Chenghui;Tang Qing;Xi Yunyun;Yang Mei;Li Tao;Huang Ying;Tao Zhu(Engineering and Research Center for Southwest Bio-pharmaceutical Resources of National Education Ministry of China, Guizhou University, Guiyang 550025;Key Laboratory of Macrocyclic and Supramolecular Chemistry of Guizhou Province, Guizhou University, Guiyang 550025;College of Tobacco Science, Guizhou University, Guiyang 550025)

机构地区：[1]贵州大学西南药用生物资源教育部工程研究中心,贵阳550025 [2]贵州大学贵州省大环化学及超分子化学重点实验室,贵阳550025 [3]贵州大学烟草学院,贵阳550025

出　　处：《有机化学》2018年第6期1394-1400,共7页Chinese Journal of Organic Chemistry

基　　金：国家自然科学基金(No.21562015);贵州省优秀青年科技人才培养计划(No.[2017]5636);贵州省科学技术基金(No.[2014]2005);贵州省普通高等学校科技拔尖人才支持计划(No.[2016]059);贵州省科技厅联合基金(No.[2014]7658);贵州大学研究生创新基金(No.2017001)资助项目~~

摘　　要：通过超分子识别设计了八元瓜环与派洛宁Y超分子荧光探针2PyY@Q[8],研究了该探针对抗癌药物拉帕替尼的识别性质.研究结果表明,该探针在水溶液中对拉帕替尼具有良好的识别能力及选择性,在一定浓度范围内其线性关系良好,其检出限为1.44×10^(-8) mol/L.细胞成像结果显示该探针在前列腺癌细胞中对拉帕替尼具有很好的响应,可用于生物细胞内拉帕替尼的识别检测.A turn-on supramolecular fluorescent probe between cucurbit[8]uril（Q[8]） and pyronine Y（Py Y）（designated 2 Py Y@Q[8]） showed a remarkable fluorescence ＂turn-on＂ response to lapatinib ditosylate（LD）. The 2PyY@Q[8] fluorescent probe can be used to detect lapatinib ditosylate with high sensitivity and selectivity with a detection limit of ca. 10-8 mol/L. A good linear relationship of emission intensity for lapatinib ditosylate at concentrations of 10×10^-8-250×10^-8mol/L was observed. The cell imaging study showed that the 2PyY@Q[8] complex could be used to image lapatinib in prostate cancer（PC3） cells, which may help to elucidate relevant biological processes at the molecular level.

关 键 词：八元瓜环 派洛宁Y 拉帕替尼 荧光探针 细胞成像 

分 类 号：O657.3[理学—分析化学] TQ460.72[理学—化学]