不育症患者精子凋亡基因Fas与FasL的表达及意义  

The expression and significance of Fas and FasL in the sperm of infertile patients

作　　者：王淑媛 黄惠伦 陈锐芳 刘宏灿 柯培锋[2] 石汉振[2] WANG Shu -yuan;HUANG Hui - lun;CHEN Rui -fang;LIU Hong - can;KE Pei - feng;Sill Han - zhen(Department of Clinical Laboratory, Haizhu Maternal and Child Health Hospital, Guangzhou 510240, Guangdong, China)

机构地区：[1]广州市海珠区妇幼保健院检验科,广东广州510240 [2]广东省中医院检验科,广东广州510120

出　　处：《广东医学》2018年第12期1827-1829,共3页Guangdong Medical Journal

基　　金：广东省科技计划项目(编号:2017ZC0387)

摘　　要：目的探讨Fas基因、FasL基因表达与精液常规主要参数以及男性不育症间的关系。方法收集120例精液标本,其中临床诊断不育者(不育年限1年以上)60例为观察组,近2年内生育体检者(备生二胎)60例为对照组,采用西班牙SCA计算机精液分析系统检测其主要参数(包括精子浓度、精子总活动率、精子前向活动率、总前向精子数);同时采用荧光相对定量PCR方法检测两组Fas基因与FasL基因表达情况。结果观察组精液体积、精子总活动率、精子前向活动率、总前向精子数降低,而Fas基因、FasL基因表达水平上调。结论 Fas、FasL基因表达上调与精子主要参数的变化有关,可能是导致部分生精功能障碍患者形成男性不育的因素。Objective To compare the difference in semen Fas/FasL gene expression of infertile patients and normal matched participants; and to investigate the correlations among the expression of Fas/FasL gene, main parameters of semen routine analysis and the male infertility. Methods Semen samples of 60 cases diagnosed as infertility patients （infertility time more than one year） and 60 cases from fertility function examination （planned to have the second child） were collected from Guangdong Provincial Hospital of Chinese Medicine and Haizhu Maternal and Child Health Hospital. Spanish SCA were used to detect the main parameters of semen （including the sperm density, total sperm activity rate, sperm progressive motility rate and total progressive sperm count）. Fas and FasL expression was measured by quantitative real - time PCR. Results The semen volume and total rate of sperm activity, sperm progressive motility and total progressive sperm count of the infertility patients were significantly reduced, while the Fas/FasL gene expression was significantly increase. Conclusion The up - regulation of Fas/FasL genes is correlated to the main parameters of sperm, which may be related factors to incomplete spermatogenesis dysfunction as well as male infertility.

关 键 词：不育症 FAS基因 FASL基因 实时荧光PCR法 

分 类 号：R698.2[医药卫生—泌尿科学]