赛格力与惠尔血动员健康供者造血干细胞效果的比较  

Comparison of the Effect of Saigeli and Huierxue on the Hematopoietic Stem Cells to Healthy Donors

作　　者：罗畅如[1] 陈伟红[1] 李明 杜新[1] 楼瑾 蔡云[1] 汪鹏程[1] 张晓瀚[1] LUO Chang-ru;CHEN Wei-hong;LI Ming;DU Xin;LOU Jin;CAI Yun;WANG Peng-cheng;ZHANG Xiao-han(Shenzhen Second People＇s Hospital, The first affiliated Hospital of Shenzhen University, Guangdong Shenzhen 518035;Shenzhen Institute of Hematology, Guangdong Shenzhen 518035)

机构地区：[1]深圳市第二人民医院,深圳大学第一附属医院,广东深圳518035 [2]深圳市血液研究所,广东深圳518035

出　　处：《深圳中西医结合杂志》2018年第7期6-9,共4页Shenzhen Journal of Integrated Traditional Chinese and Western Medicine

基　　金：深圳市知识创新计划基础研究资金资助课题(JCYJ20140414170821270);深圳市骨髓移植技术临床应用公共服务平台资金资助课题(S2015003610009)

摘　　要：目的:回顾性比较国产粒细胞集落刺激因子(G–CSF)赛格力与进口G–CSF惠尔血对健康供者外周血造血干细胞的动员效果。方法:供者应用赛格力或惠尔血4.2~12.8μg·kg^(-1)·d^(-1)连续5 d皮下注射,若第4天白细胞低于30×10~9·L^(-1),第4.5天开始采集前2 h加用G–CSF 5μg·kg^(-1)皮下注射,用COBE Spectra血细胞分离机采集外周血造血干细胞,若采集数量不够,第5.5天再采集1次。并观察供者动员、采集时出现的不良反应。结果:赛格力组与惠尔血组所用剂量,分别为(7.6±1.6)μg·kg^(-1)·d^(-1),(5.4±0.7)μg·kg^(-1)·d^(-1),组间比较,差异具有统计学意义(P<0.05)。赛格力组与惠尔血组动员后白细胞均升高,分别为(43.3±11.1)×10~9·L^(-1),(45.1±7.5)×10~9·L^(-1),组间比较,差异无统计学意义(P>0.05)。赛格力与惠尔血对供者外周血造血干细胞的影响,赛格力组有2例供者需采集2次,两组结果分别为采集物单个核细胞(MNC)·kg^(-1)(9.3±4.4)×10~8·kg^(-1),MNC·kg^(-1)(8.9±6.2)×10~8·kg^(-1);CD34^+·kg^(-1)(6.9±5.3)×10~6·kg^(-1),CD34^+·kg^(-1)(8.3±7.3)×10~6·kg^(-1),组间比较,差异无统计学意义(P>0.05)。两种动员剂对供者不良反应无显著差异。结论:赛格力动员效果略差于惠尔血,但通过增加赛格力剂量、采集干细胞次数,两者均能成功的动员健康供者。赛格力能可靠用于健康供者的动员。Objective To retrospectively compare the mobilization effects of Domestically–produced granulocyte colony–stimulating factor（G–CSF）Saigeli and imported G–CSF Huierxue on peripheral hematopoietic stem cells in healthy donors.Methods The donor was injected subcutaneously with Saigeli or Huierxue 4.2～12.8μg/（kg·d）for 5 days continuously.If white blood cell count dropped below 30×10^9/L on day 4.5μg/kg of G–CSF would be injected subcutaneously 2 hours before day 4 and a half;a COBE Spectra blood cell separator would be used to collect peripheral hematopoietic stem cells.In the event that the quantity collected was not sufficient,an additional sample would be collected on day 5 and a half.Observe the side reaction of the donors during the collecting and mobilizing.Results The doses used in the Saigle and Whirl Blood groups were（7.6±1.6）μg·kg^-1·d^-1and（5.4±0.7）μg·kg^-1·d^-1 respectively.Statistical significance（P〈0.05）.The leukocytes were increased after mobilization in the Saigle group and the Huier group（43.3±11.1）×10^9·L^-1,（45.1±7.5）×10^9·L^-1.There was no significant difference between the two groups（P〈0.05）.The effect of Segral and Whirlpool blood on peripheral blood hematopoietic stem cells from donors was obtained from 2 donors in the Segrel group.The results were obtained from mononuclear cells（MNC）·kg^-1（9.3±4.4）×10^8·kg^-1,MNC·kg^-1（8.9±6.2）×10^8·kg^-1;CD34^＋·kg^-1（6.9±5.3）×10^6·kg^-1,CD34^＋·kg^-1（8.3±7.3）×10^6·kg^-1,there was no significant difference between groups（P〈0.05）.There was no significant difference in donor adverse reaction between the two mobilizers.Conclusions The effect of Saigeli mobilization is slightly worse than that of Huierxue,but both of them can successfully mobilize healthy donors by increasing the dose of Saigeli and collecting the number of stem cells.Saigeli can be used reliably for the mobilization of healthy donors.

关 键 词：粒细胞集落刺激因子 造血干细胞 赛格力 惠尔血 

分 类 号：R45[医药卫生—治疗学]