HFSC标记在阿尔巴斯绒山羊毛囊及毛囊干细胞中的表达  

Expression of HFSC Marker in the Arbas Cashmere Goat Hair Follicle and Hair Follicle Stem Cells

作　　者：乃门塔娜 张燕军[1] 刘东军[2] 李金泉[1] NIMANTANA;ZHANG Yan-jun(College of Animal Science, Inner Mongolia Agricultural University, Biology and Biotechnology of Ministry of Education, LIU Dong-jun2 LI Jin-quan1 Huhehot 010018;Key Laboratory of Mammalian Reproductive Inner Mongolia University, Huhehot 010070)

机构地区：[1]内蒙古农业大学动物科学学院,呼和浩特010018 [2]内蒙古大学哺乳动物生殖生物学及生物技术教育部重点实验室,呼和浩特010070

出　　处：《生物技术通报》2018年第5期201-205,共5页Biotechnology Bulletin

基　　金：中国博士后科学基金资助项目(2017M613263XB)

摘　　要：旨在检测最广泛应用的毛囊干细胞标记在阿尔巴斯绒山羊毛囊组织中的表达情况,为今后内蒙古绒山羊毛囊组织学和细胞学领域相关研究提供依据。以阿尔巴斯绒山羊为研究对象,对其进行Krt15、Krt19、CD34和Sox9等的冰冻切片和细胞免疫组化实验。以上4个标记均在外根鞘中表达;Krt15、Krt19在隆突部表达,在隆突部下端不连续表达;CD34在隆突部和隆突部下端都有较强的表达;Sox9主要集中在隆突部表达。Krt15、Krt19、CD34和Sox9共同表达的部位为毛囊隆突部,因此可结合表达部位特征选用为阿尔巴斯绒山羊毛囊隆突部来源的毛囊干细胞鉴定标记。This study is aimed to detect the expression of widely-used hair follicle stem cell（HFSC）markers in the hair follicle of Arbas Cashmere goat,and to provide experimental evidence for further research on histology and cytology of hair follicle in Inner Mongolia Cashmeregoat. Using Arbas Cashmere goat as study object,frozen section and immunohistochemistry of Krt15,Krt19,CD34 and Sox9 were performed.Results showed that the above-mentioned four markers all expressed in the outer root sheath;Krt15 and Krt19 positively expressed in the bulgeregion and non-contiguously in the suprabulbar region;CD34 had a strong expression in the bulge and suprabulbar region;Sox9 expressionfocused in the bulge region. Conclusively,Krt15,Krt19,CD34 and Sox9 all expressed in the bulge region of the hair follicle,thus they can be used as special markers of Arbas Cashmere goat hair follicle stem cells from bulge region according to their expressed location.

关 键 词：阿尔巴斯绒山羊 冰冻切片 细胞免疫组化 鉴定标记 

分 类 号：S827[农业科学—畜牧学]