灵芝多糖对人肝癌细胞HepG2增殖及体外迁移的影响  

The inhibiting effects of Ganoderma lucidum polysaccharides on proliferation and migration of HepG2 liver cancer cells

作　　者：陈源红[1] 邹佳峻[2] 李松波[1] 黄干荣[1] 郑紫菱 董宇曦 杨梦夕 罗艳红[2] CHEN Yuan - hong;ZOU Jia -jun;LI Song - bong;HUANG Gan - rong;ZHENG Zi - ling;DONG Yu - xi;YANG Meng - xi;LUO Yah - hong(Teaching and Research Section of Pathogeny Biology, Youjiang Medical University for Nationalitie;Baise 533000, Guangxi, Chin)

机构地区：[1]右江民族医学院病原生物学与免疫学教研室,广西百色533000 [2]右江民族医学院检验学院,广西百色533000 [3]右江民族医学院口腔学院,广西百色533000

出　　处：《广东医学》2018年第11期1625-1628,共4页Guangdong Medical Journal

基　　金：国家自然科学基金资助项目(编号:81660642);广西高校中青年教师基础能力提升项目(编号:2017KY0518)

摘　　要：目的体外检测灵芝多糖对人肝癌细胞株Hep G2迁移的影响,体内检测灵芝多糖对荷瘤小鼠的增殖抑制作用。方法 Transwell法检测灵芝多糖组(加灵芝多糖960μg/m L)与阴性对照组(加等体积培养液)的Hep G2细胞迁移数,ELISA法分别检测两组的血管内皮生长因子(VEGF)蛋白含量,取细胞密度为1×107个/m L的对数生长期Hep G2细胞,于BALB/c裸鼠左侧腋下行无菌皮下接种,建立肝癌移植瘤BALB/c裸鼠模型,并用灵芝多糖作用于人皮下Hep G2移植瘤模型。结果灵芝多糖组的Hep G2细胞迁移数较阴性对照组明显减弱,差异有统计学意义(P<0.05),灵芝多糖治疗组12、24 h的侵袭抑制率分别为31.7%、64.0%,与阴性对照组比较有统计学意义(P<0.05),并具有时间依赖性;灵芝多糖组与阴性对照组相比,VEGF蛋白含量显著下降,具有时间依赖性;灵芝多糖可体内抑制肝癌皮下移植瘤,1周给药、2周给药的抑瘤率分别为18.98%、37.33%,与阴性对照组比较差异均有统计学意义(P<0.05),并具有时间依赖性。结论灵芝多糖对Hep G2细胞的增殖、迁移有抑制作用,其迁移抑制通过下调VEGF蛋白含量而实现。Objective To test the effect of ganoderma lucidum polysaccharides （GLP） on migration of HepG2 liver cancer cells in vitro, as well as to detect the inhibiting effect on the cancer cells of tumor - bearing mice. Methods Transwell method was used to test the migration ability of HepG2 cells in the GLP treatment group and Control Group. ELISA method was used to test the vascular endothelial growth factor （VEGF） protein of HepG2 cells in both groups. HepG2 cells （107/mL） were implanted into the left oxterof BALB/c naked mice. The inhibition of tumor growth was used to test the proliferation of the cancer cells. Results Compared with control group, the invasion inhibition rates were 31.7% and 64. 0% after treatment with GLP for 12 hours and 24 hours, respectively. The migration ability of HepG2 cells in the treatment group was significantly reduced n a time - dependent manner （ P 〈 0. 05 ）. Compared with control group, the expression of VEGF protein in the treatment group was significantly reduced （ P 〈 0. 05 ）. GLP inhibited the growth of transplanted hepatocellular carcinoma on nude mice, while the anti - tumor rates were 18.98% and 37. 33% after treat- ment with GLP for one week and two weeks, respectively; significantly differed from control group. Conclusion GLP can inhibit the proliferation and migration of HepG2 cells by down - regulating VEGF proteins.

关 键 词：灵芝多糖 HEPG2 VEGF 增殖 迁移 

分 类 号：R285[医药卫生—中药学]