动态三维超声造影定量分析对裸鼠皮下种植瘤不全微波消融的疗效评估  

Dynamic three-dimensional contrast-enhanced ultrasound for treatment assessment of incomplete microwavecoagulation on xenograft tumors in nude mice

作　　者：曹佳颖 毛枫 范培丽 王文平 Cao Jiaying;Mao Feng;Fan Peili;Wang Wenping(Department of Ultrasound, Zhongshan Hospital, Fudan University, Shanghai 200032, Chin)

机构地区：[1]复旦大学附属中山医院超声科,上海200032 [2]上海市影像医学研究所

出　　处：《中华超声影像学杂志》2018年第6期529-534,共6页Chinese Journal of Ultrasonography

基　　金：国家自然科学基金（81571676）;上海市医学领军人才;上海市科学与技术委员会基金（16411968400）;上海市卫生与计划生育委员会基金面上项目（201540077）

摘　　要：目的评价动态三维超声造影（3D-CEUS）定量分析技术对于裸鼠皮下种植瘤的不全微波热消融治疗后血流动力学变化的评估能力。方法建立BALB／c裸鼠HepG2细胞皮下种植瘤模型。模型动物分为治疗组（16例）和对照组（16例），在肿瘤生长至最大径约0．8-1．0cm时，采用微波对肿瘤进行不全热消融。并在治疗前1d及治疗后第3d、第7d、第14d进行常规超声及动态3D-CEUS检查。通过拟合，得出肿瘤组织的时间-强度曲线及定量参数，包括峰值强度（PI）、达峰时间（TTP）、平均渡越时间（MTT）、上升支斜率（s）、曲线下面积（AUC）、上升段曲线下面积（AwI）和下降段曲线下面积（AWO）。结果在治疗后第3d和第14d时，两组之间PI、S、AUC、Awo比较，差异均有统计学意义（P=0．029），而其余参数比较差异均无统计学意义（P〉0．05）。在治疗前1d和治疗后第7d时，各个参数比较差异均无统计学意义（P〉0．05）。结论应用动态3D-CEUS定量分析方法可反映裸鼠皮下种植瘤的治疗后状态，有助于评估疗效。Objective To evaluate the feasibility of quantitative analysis of dynamic 3-dimensional contrast-enhanced ultrasound （3D-CEUS） for treatment effects of microwave coagulation on xenograft tumors in nude mice. Methods BALB/c nude mice transplanted of HepG2 ceils were divided into treatment group（ n = 16） and control group（ n = 16）. The tumors were treated by incomplete microwave coagulation in the treatment group when their maximum diameters were about 0.8-1.0 cm. The baseline ultrasound and dynamic 3D-CEUS were performed on 1 d before treatment, 3 d after treatment, 7 d after treatment and 14 d after treatment. Time-intensity curves of the tumors were obtained by fitting. The quantitative parameters were also obtained, including peak intensity （PI）, time to peak （TTP）, mean transit time （MTT）, slope （S）, area under the curve （AUC）, area under the curve of wash-in （AWl）, and area under the curve of wash-out （AWO）. Results There were significant differences （ P = 0.029） between the two groups in PI, S, AUC, AWO on 3 d and 14 d. The differences in other parameters were not statistically significant （ P 〉0.05）. On 1 d before treatment and 7 d after treatment, none of the parameters had statistically significant difference （ P 〉0.05）. Conclusions The quantitative analysis of dynamic 3D-CEUS

关 键 词：超声造影 三维 小鼠 微波 消融技术 

分 类 号：R445.1[医药卫生—影像医学与核医学] R73[医药卫生—诊断学]