牛肠道病毒北京株的分离鉴定及全基因组分析  

Isolation and identification of bovine enterovirus Beijing isolate and sequence analysis of whole genome of the isolate

作　　者：张姗[1] 李晓轩 侯绍华[2] 鑫婷[2] 林湛椰 袁万哲[1,4] 孙继国 ZHANG Shan;LI Xiao-xuan;HOU Shao-hua;XIN Ting;LIN Zhan-ye;YUAN Wan-zhe;SUN Ji-guo(College of Animal Medicine ,Agriculture University of Hebei, Baoding 071001, China;Beijing Institute ofA nimal Science and Veterinary Medicine ,Chinese Academy of Agricultural Sciences ,Beijing 100193, China;Veterinary Bureau, Ministry of Agticulture of the People＇s Republic of China,Beijing 100125 ,China;Hebei Engineering and Technology Research Center of Veterinary Biotechnology , Baoding 071001, China)

机构地区：[1]河北农业大学动物医学院,河北保定071001 [2]中国农业科学院北京畜牧兽医研究所,北京100193 [3]中华人民共和国农业部兽医局,北京100125 [4]河北省兽医生物技术工程技术研究中心,河北保定071001

出　　处：《中国兽医科学》2018年第7期844-850,共7页Chinese Veterinary Science

基　　金：国家重点基础研究发展计划项目(2016YFD0500902)

摘　　要：采用RT-PCR方法从北京某牛场有腹泻症状牛的粪便样品中检测到牛肠道病毒（bovine enterovirus,BEV）,分离出1株病毒并将其命名为BJ101。然后,通过电镜观察、全基因组序列测定和同源性分析,对该毒株进行了验证。结果,BJ101分离株在MDBK细胞上的TCID50为1×10^-6.42/0.1 m L,电镜观察到病毒颗粒直径为25～30 nm,无囊膜,呈典型的小RNA病毒粒子形态;BJ101株的基因组长度为7 409 bp,其中5′非编码区（UTR）长812 bp,3′UTR长72 bp,病毒基因组开放阅读框（ORF）全长6 525 bp。对全基因组序列进行比对分析显示,在5′UTR区、编码区和3′UTR区,BJ101分离株与BEV E2型毒株的核苷酸相似性最高。基于VP1基因的系统进化树表明,BJ101株是BEV E2型的成员。上述结果丰富了国内牛肠道病毒的基因库,为BEV的诊断及预防奠定了良好的基础。A strain of bovine enterovirus（bovine enterovirus,BEV）was isolated from the fecal samples of cattle affected with diarrhea in Beijing and was identified as a strain of bovine enterovirus by RT-PCR,and was named BJ101 isolate.The full-length genome of BJ101 isolate was sequenced and its molecular characteristic was analyzed. In result,the TCID50 of the isolate was 1×10^-6.42/0.1 mL and the virion was observed as non-enveloped particles about 25-35 nm in diameter.The full genomic size of BJ101 was 7 409 bp,which contained 5′UTR（812 bp）,the open reading frame（ORF,6 525 bp）and 3′UTR（72 bp）.The sequence alignments showed that the BJ101 strain shared the highest nucleotide identities with the strains of BEV E2 in the 5′UTR,the coding region and the 3′UTR.The phylogenetic analysis based on the VP1 region shows that the BJ101 strain belongs to BEV E2 sub-genotype.The above-mentioned results have enriched the gene pool of bove enterovirus and laid a good foundation for the diagnosis and prevention of bovine enterovirus.

关 键 词：牛肠道病毒 分离 基因组 比对分析 

分 类 号：S852.653[农业科学—基础兽医学]