NAC在镉致PC12细胞凋亡线粒体途径中的保护效应  

Protective effect of NAC on Cd-induced mitochondrial apoptotic pathway in PC12 cells

作　　者：袁燕[1,2] 江辰阳 达剑森[3] 陈洁 赵世文 邹辉 顾建红[1,2] 刘学忠 卞建春[1,2] 刘宗平 YUAN Yan;JIANG Chen-yang;DA Jian-sen;CHEN Jie;ZHAO Shi-wen;ZOU Hui;GU Jian-hong;LIU Xue-zhong;BIAN Jian-chun;LIU Zong-ping(College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China;Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China;The Animal Husbandry and Veterinary Station of Hanjiang District of Yangzhou , Yangzhou 225002, China)

机构地区：[1]扬州大学兽医学院,江苏扬州225009 [2]江苏高校动物重要疫病与人兽共患病防控协同创新中心,江苏扬州225009 [3]扬州市邗江区畜牧兽医站,江苏扬州225002

出　　处：《中国兽医科学》2018年第7期924-930,共7页Chinese Veterinary Science

基　　金：国家自然科学基金资助项目(31772808);中国博士后科学基金资助项目(2015M581874);江苏省博士后科研资助计划项目(1501072A);国家重点研发计划支持项目(2016YFD0501208);江苏高校优势学科建设工程资助项目(PAPD)

摘　　要：为研究N-乙酰半胱氨酸（NAC）在镉致PC12细胞凋亡线粒体途径中的保护作用,本试验用10mol/L醋酸镉和100 mol/L NAC单独或联合处理PC12细胞24 h后,用MTT法检测细胞存活率;用流式细胞术检测细胞凋亡率;Western-blot法检测细胞色素C（cytochrome c,Cyt C）的释放以及Bcl-2、Bax、Cleaved caspase-3和Cleaved PARP蛋白的表达量。结果,NAC能显著或极显著抑制镉引起的细胞存活率下降,凋亡率上升,Cyt C从线粒体释放进入胞浆中,Bcl-2/Bax比值下降,Cleaved caspase-3和Cleaved PARP蛋白的表达量提高（P〈0.05或P〈0.01）。上述结果说明,NAC在镉致PC12细胞凋亡线粒体途径中具有保护作用。To investigate the protective effect of N-acetylcysteine（NAC） on mitochondrial apoptotic pathway induced by cadmium（Cd）,PC-12 cells were exposed to Cd（10 mol/L） and/or 100 mol/L NAC for 24 h.Then,cell viability was assayed by MTT assay,cell apoptotic rate was measured by flow cytometry,the release of cytochrome c（Cyt C） and the protein levels of Bcl-2,Bax,cleaved caspase-3 and cleaved PARP were measured by Western-blot.In result,Cd-induced decrease of cell viability, increase of apoptotic rate,release of Cyt C,reduction of the B-cell lymphoma 2（Bcl-2）/Bcl-2 associate X protein（Bax） ratio,as well as increase of Cleavedcapase-3 and Cleaved PARP protein levels were inhibited by NAC significantly（P〈0.05 or P〈0.01）.These findings suggest that NAC can provides protection against Cdinduced apoptosis via a mitochondrial apoptotic pathway in PC12 cells.

关 键 词：镉 N-乙酰半胱氨酸(NAC) PC12细胞 凋亡 线粒体途径 

分 类 号：S856.5[农业科学—临床兽医学]