基于荧光共振能量转移的金纳米粒子/碳量子点荧光纳米探针检测精氨酸  

Detection of Arginine by AuNPs/CQDs Nanoprobes Based on Fluorescence Resonance Energy Transfer Effect

作　　者：邹小波[1] 史永强 郑悦[2] 石吉勇[1] 胡雪桃 蒋彩萍 黄晓玮[1] 徐艺伟 ZOU Xiao-Bo1 , SHI Yong-Qiang1 , ZHENG Yue2, SHI Ji-Yong1, HU Xue-Tao1, JIANG Cai-Ping1, HUANG Xiao-Wei1, XU Yi-Wei1(1School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China; 2 Chinese Academy of Agricultural Mechanization Sciences, Beijing 100083, Chin)

机构地区：[1]江苏大学食品与生物工程学院,镇江212013 [2]中国农业机械化科学研究院,北京100083

出　　处：《分析化学》2018年第6期960-968,共9页Chinese Journal of Analytical Chemistry

基　　金：国家重点研发计划项目(No.2016YFD0401104);国家科技支持计划项目(No.2015BAD17B04);国家自然科学基金项目(No.31671844)资助~~

摘　　要：利用碳量子点(CQDs)和金纳米粒子(Au NPs)间的荧光共振能量转移(FRET)效应成功实现了精氨酸的快速检测。采用一步微波辅助法合成具有优良荧光性能的碳量子点(CQDs),并对Au NPs/CQDs复合材料进行相应表征,分析了其猝灭和检测机制。对Au NPs/CQDs的添加量、p H值和反应时间进行了优化。在优化条件下,将此荧光体系用于精氨酸含量的检测,结果表明,荧光强度和精氨酸浓度在0.1~10.0μmol/L范围内呈良好的线性关系(R^2=0.993),检出限为5.8 nmol/L。采用本方法测定了葡萄汁中精氨酸的含量,回收率为105.4%~110.8%,表明本方法可用于果汁中精氨酸的实际检测。A novel method for rapid detection of arginine based on fluorescence resonance energy transfer effect（ FRET） between carbon quantum dots（ CQDs） and gold nanoparticles（ Au NPs） was developed.Firstly,the CQDs with excellent fluorescence properties were synthesized by one-step microwave assisted method. The Au NPs/CQDs composites were characterized and their quenching mechanism was analyzed. Then the amount of Au NPs/CQDs,the p H value and the reaction time were optimal. Under the optimum conditions,the fluorescence system was used to detect the content of arginine,showing a good linear relationship（ R^2= 0.993） between fluorescence intensity and concentration of arginine in the range of 0.1-10.0 μmol/L,and the detection limit was 5.8 nmol/L. Finally,the content of arginine in grape juice was determined by this method with recoveries of 105. 4%-110. 8%,which indicated that the proposed FRET system had the potential for practical detection of arginine in fruit juice.

关 键 词：精氨酸 荧光探针 荧光共振能量转移 碳量子点 金纳米粒子 

分 类 号：O657.3[理学—分析化学] TS255.44[理学—化学]