Goldeneye 20A试剂盒检测发现TPOX基因座三等位基因一例  

A case of tri-allele in TPOX was detected using Goldeneye 20A Kit for the first time

作　　者：周单华 胡锡阶[1,2] 章涛[1,2] 万卫红[1,2] 余舰[2] 刘祖林[1,2] 余丽梅[1,2] ZHOU Danhua;HU Xijie;ZHANG Tao;WAN Weihong;YU Jian;LIU Zulin;YU Limei(Key Laboratory of Cell Engineering in Guizhou Province, Guizhou Province, Zunyi 563003, China;Centre ofForensic Medicine, the Affiliated Hospital of Zunyi Medical University, Guizhou Province, Zunyi 563003, China)

机构地区：[1]遵义医学院附属医院贵州省细胞工程重点实验室,贵州遵义563000 [2]遵义医学院附属医院法医鉴定中心,贵州遵义563000

出　　处：《中国医药导报》2018年第14期146-148,共3页China Medical Herald

基　　金：贵州省生物治疗人才基地定向招标课题资助项目(黔人领发[2013]15号)

摘　　要：本文报道了二联体检案中孩子的TPOX基因座上检测到三等位基因1例。采用Goldeneye 20A试剂盒检测,3500DX遗传分析仪电泳,Genemapper基因分型软件基因分型,确认其基因分型为(8,9,11)。亲子鉴定中检测到三等位基因时,在排除污染前提下,至少需两种试剂盒检测确认,以保证鉴定结果及PI计算的可靠性。为提高检案的准确性,需谨慎对待三等位基因的检测、峰型判断、分析和确认,进而也可为产生三等位基因的可能机制提供基础信息。This article reports one case of tri-allele detected in the TPOX locus of a child in duos. The Goldeneye20 A kit was used for detecting. The 3500 DX genetic analyzer was used for electrophoresis and Genemapper genotyping software was used confirm its genotyping（8, 9, 11）. When the tri-allelic gene is detected in the paternity test, under the premise of eliminating contamination, at least two kits are required to confirm the test to ensure the reliability of the identification and PI calculation. In order to improve the accuracy of the case report, the tri-allele test, the peak type analysis, the classification and judgment and the confirmation should be handled with care so that provides basic information for the analysis of the possible mechanisms of tri-allele.

关 键 词：三等位基因 Goldeneye 20A试剂盒 亲子鉴定 TPOX 

分 类 号：DF795.2[医药卫生—法医学]