DP_2拮抗剂抑制哮喘模型小鼠气道炎症的作用机制及与激素的比较  

Mechanism of DP_2 antagonist on inhibiting airway inflammation in asthma mice model and the comparison with inhaled corticosteroid

作　　者：董樑[1] 龚益[1] 朱柠[1] 张媛媛[1] 夏敬文[1] 陈小东[1] Dong Liang, Gong Yi, Zhu Ning, Zhang Yuanyuan, XiaJingwen, Chen Xiaodong.(Department of Respiratory Medicine, Huashan Hospital, Fudan University, Shanghai200040, Chin)

机构地区：[1]复旦大学附属华山医院呼吸科,上海200040

出　　处：《中华肺部疾病杂志（电子版）》2018年第2期172-175,共4页Chinese Journal of Lung Diseases(Electronic Edition)

基　　金：上海市科委科研计划项目(14411951100)

摘　　要：目的探讨DP2拮抗剂抑制哮喘模型小鼠气道炎症的作用机制,并比较其同吸入激素的差异。方法 SPF级BALB/c小鼠24只,随机分为正常对照组(A组)、哮喘组(B组)、吸入激素干预组(C组)和DP2拮抗剂干预组(D组),每组6只,用卵白蛋白(OVA)雾化诱喘。应用Pro Cyte DxTM全自动血细胞分析仪进行支气管肺泡灌洗液细胞分类计数,Western blot方法检测肺组织DP1和DP2蛋白的表达。结果与B组相比,C、D两组的嗜酸性粒细胞计数、DP1和DP2蛋白表达水平均显著降低(P<0.01)。D组的DP2蛋白水平较A组显著下降(P<0.01),C组的DP2蛋白水平较A组亦有下降(P<0.05),C、D两组之间比较有统计学异常(P<0.05)。DP1的蛋白表达水平在A、C、D组之间无统计学差异。结论 DP2拮抗剂能通过抑制其蛋白表达水平从而减少气道的嗜酸性粒细胞浸润,达到抑制哮喘模型小鼠气道炎症的作用。DP2拮抗剂对DP2蛋白表达的抑制作用强于吸入激素,或可作为新的药物用于支气管哮喘的治疗。Objective To understand the mechanism of DP2 antagonist on inhibiting airway inflammation in asthma mice model and to compare the differences between the DP2 antagonist and inhaled corticosteroid. Methods 24 SPF BALB/c mice were randomly divided into normal control group（group A）,asthma group（group B）,inhalation corticosteroid group（group C） and DP2 antagonist group（group D）,with6 rats in each group. Asthma was induced by ovalbumin（OVA） challenge. Bronchoalveolar lavage fluid（BALF） was analyzed by the Pro Cyte Dx TM automatic blood cell analyzer. The expression of lung tissue DP1 and DP2 protein was detected by Western blot method. Results Compared with group B,eosinophils count,DP1 and DP2 protein levels were significantly reduced in groups C and D（P〈0.01）. The level of DP2 protein in groups C and D was significantly lower than that in group A（P〈0.01）. There was statistical difference between group C and D（P〈0.05）. No significant difference in the expression level of DP1 was detected between group A,C and D. Conclusion DP2 antagonist can control airway inflammation in asthma mice model by inhibiting the expression of the DP2 protein and decreasing the infiltration of eosinophils. Its effect on the expression of DP2 protein is stronger than that of inhaled corticosteroid. DP2 antagonist probably can be used as a new drug for the treatment of asthma.

关 键 词：支气管哮喘 前列腺素D2受体 吸入糖皮质激素 

分 类 号：R563[医药卫生—呼吸系统]