基于萘酰腙类锌离子增强型荧光探针的合成及光谱性能  

Synthesis and Spectral Properties of Zinc Ion-enhanced Fluorescence Probe Based on Naphthoyl Acylhydrazone

作　　者：吴红梅[1] 郭宇[1] 曹建芳 吴中立[2] WU Hong-mei1, GUO Yu1, CAO Jian-fang1, WU Zhong-li2(1. School of Chemical and Environmental Engineering？ Liaoning University of Technology,Jinzhou 121001, China2. College of Mechanical Engineering and Automation, Liaoning University of Technology, Jinzhou 121001, Chin)

机构地区：[1]辽宁工业大学 化学与环境工程学院，辽宁 锦州 121001 [2]辽宁工业大学 机械工程与自动化学院，辽宁 锦州 121001

出　　处：《发光学报》2018年第5期621-626,共6页Chinese Journal of Luminescence

基　　金：国家自然科学基金(21601075,21606118); 辽宁省自然科学基金(2015020249)资助项目.

摘　　要：设计合成了一种简单的酰腙类结构的增强型荧光探针HM,实现了对锌离子(Zn^(2+))的高选择性识别。运用ESI-MS质谱、荧光光谱和紫外-可见光谱等手段研究了探针HM对Zn^(2+)的识别过程。紫外光谱测试表明,当向探针HM中加入Zn^(2+)后,386 nm处的吸收峰逐渐减弱,在420 nm处出现了新的吸收峰,并且强度逐渐增大,直至达到平衡,等吸收点为396 nm。荧光光谱分析表明,探针HM能够高选择性地识别Zn^(2+)。在发射波长510 nm处的荧光增强2.5倍,最低检出限为1.0×10^(-5)mol/L、量子产率为0.02。该识别过程为PET(光诱导电子转移机理)和CHEF(螯合荧光增强机理)共同作用的结果。通过电喷雾质谱和Job's plot实验证明探针HM与Zn^(2+)以1∶1配位,平衡常数(K)达到4.05×10~6L·mol^(-1)。A simple fluorescent probe HM based on acylhydrazone structure was synthesized for recognition of zinc ion（Zn2＋）.The recognition process of Zn2＋was studied by ESI-MS,fluorescence spectroscopy and UV-Vis spectroscopy.UV-Vis titration test shows that the addition of Zn2＋into HM aqueous solution caused gradually drop of the absorption peak at 386 nm.At the same time,a new absorption peak at 420 nm appeared and the intensity gradually increased until equilibrium.A sharp isosbestic point at 396 nm was obtained.Florescence spectroscopy indicates that the fluorescent probe HM exhibites high selectivity for detection of Zn2＋.The fluorescence intensity was increased by 2.5 times at excited wavelength of 510 nm.The lowest detection limit of HM for Zn2＋is up to1.0×10-5mol/L and the fluorescence quantum yield is 0.02.The recognition process is presumed to be caused by PET（photoinduced electron transfer）process combining with CHEF（Chelation enhanced fluorescence）mechanism.The Job＇s plot and ESI-MS results demonstrate a 1∶1 stoichiometric host-guest complex for probe molecular HM and Zn2＋,with the association constant being calculated as K = 4. 05 × 106 L·mol-1.

关 键 词：锌离子 荧光探针 酰腙 识别 

分 类 号：O433.5[机械工程—光学工程]