松墨天牛幼虫肠道纤维素降解细菌的分离与鉴定  

Isolation and identification of cellulolytic bacteria associated with the gut of Monochamus alternatus larvae

作　　者：胡霞[1] 傅慧静 李俊楠[1] 林中平 张飞萍[1] HU Xia1, FU Huijing1, LI Junnan1, LIN Zhongping2, ZHANG Feiping1(1.College of Forestry,Fujian Agriculture and Forestry University, Fuzhou,Fujian 350002,China;2.Forestry Bureau of Fuzhou, Fuzhou,Fujian 350000,Chin)

机构地区：[1]福建农林大学林学院,福建福州350002 [2]福州市林业局,福建福州350000

出　　处：《福建农林大学学报（自然科学版）》2018年第3期322-328,共7页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：国家林业公益性行业科研专项资助项目(201304401);福建省自然科学基金青年项目(2016J05064)

摘　　要：为揭示松墨天牛幼虫肠道木质纤维素降解细菌群落的结构和功能,采用羧甲基纤维素钠为唯一碳源,结合刚果红法筛选肠道纤维素降解细菌,共获得154株肠道纤维素降解细菌,分别隶属于变形菌门、厚壁菌门和拟杆菌门细菌的8个菌属、10个菌种.其中噬纤维细菌科细菌Siphonobacter aquaeclarae表现为最优势纤维素降解菌,占肠道纤维素降解细菌群落的31.8%,其次为沙雷氏菌Serratia marcescens,占20.1%,其它依次为芽孢杆菌(Bacillus),克雷伯氏杆菌(Klebsiella),鞘脂菌(Sphingobium),丛毛单孢菌(Comamonas)和黄色单胞菌(Lysobacter).The cellulolytic bacterial community in the intestine of Monochamus alternatus larvae was investigated in the liquid media with the unique carbon sources of sodium carboxymethylcellulose or filter paper. The results showed that a total of 154 cellulolytic bacteria belong to Proteobacteria,Firmicutes and Bacteroidetes were isolated and 10 species in 8 genera were identified. The commonest species was Siphonobacter aquaeclarae which accounted for 31.8% in the cellulolytic bacterial community,followed by Serratia marcescens which accounted for 20.1%. The other species in Bacillus,Klebsiella,Sphingobium,Comamonas and Lysobacter were also found.

关 键 词：松墨天牛 肠道细菌 群落结构 纤维素酶 细菌鉴定 

分 类 号：S763.3[农业科学—森林保护学]