巨菌草不同生长时期根、茎、叶内生细菌组成的多样性及差异  

Composition diversity and differences of endophytic bacteria in root,stem and leaf at different growth stages of Pennisetum sp.

作　　者：林标声 宋昭昭[1,3] 张丽丽 范锦琳[1,3] 林占熺 LIN Biaosheng1,2, SONG Zhaozhao1,3, ZHANG Li l i1,3, FAN Jin lin1,3, LIN Zhanxi1,3(1.College of Life Science,Fujian Agriculture and Forestry University, Fuzhou,Fujian 350002, China;2.College of Life Science,Longyan University, Longyan,Fujian 364012, China; 3.National Engineering Research Center of Juncao, Fuzhou,Fujian 350002, Chin)

机构地区：[1]福建农林大学生命科学学院,福建福州350002 [2]龙岩学院生命科学学院,福建龙岩364012 [3]国家菌草工程技术研究中心,福建福州350002

出　　处：《福建农林大学学报（自然科学版）》2018年第3期352-360,共9页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：菌草生态产业协同创新攻关课题(JCXTGG01);福建省大学生创新创业训练计划项目(G20171007);福建省科技厅重大专项项目(2014NZ2002-1)

摘　　要：以巨菌草不同生长时期的根、茎、叶为研究对象,采用Illumina Miseq高通量测序技术分析其内生细菌的组成.结果表明:巨菌草不同生长时期根、茎、叶内生细菌组成的多样性及丰度均有较大的差异.从门到属的水平看,巨菌草内生菌群组成均为拔节期较高,在同一生长时期为根>茎>叶;蓝藻菌门(Cyanobacteria)和变形菌门(Proteobacteria)为主要菌群门类,罗尔斯顿菌属(Ralstonia)和乳球菌属(Lactococcus)为核心属.Heatmap聚类和主成分分析表明,根部、叶部来源的细菌菌群组成较为接近,茎部来源的菌群与根部、叶部有交叉.典范对应分析表明,各来源样本菌群的组成主要受温度的影响,其次为湿度和p H.The composition diversity and differences on endophytic bacteria in root,stem and leaf at different growth stages of Pennisetum sp. were analyzed by high-throughput sequencing technology based on Illumina Miseq platform. The results revealled that there were great differences in composition and abundance of endophytic bacteria in roots,stems and leaves at different growth stages of Pennisetum sp. From phylum to genus level,the abundance of Pennisetum sp. endophytic bacteria was the highest in jointing period,and it was rootstemleaf within the same growth period. Cyanobacteria and Proteobacteria were the main phyla,and Ralstonia and Lactococcus were the dominant genus. Heatmap and principal component analysis showed that bacteria community composition from root and leaf was close to each other,while those from stem had a cross with root and leaf. Canonical correspondence analysis showed that the composition of bacteria community was mainly affected by environmental temperature,followed by moisture and p H.

关 键 词：巨菌草 高通量测序 内生细菌 多样性 差异 

分 类 号：S544.9[农业科学—作物学]