声源定位过程中低位核团神经元的形态及功能特性比较  

Comparison of morphology and functional characteristics of brainstem nuclei during the process of sound source localization

作　　者：傅鸣宇[1] 王宁黔[1] 肖中举[1] FU Mingyu, WANG Ningqian, XIAO Zhongju(School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, Chin)

机构地区：[1]南方医科大学基础医学院,广州510515

出　　处：《中国科技论文》2018年第6期636-641,共6页China Sciencepaper

基　　金：高等学校博士学科点专项科研基金资助项目(20124433120008);广东省医学科学技术研究基金资助项目(WSTJJ2014010621010-2197510241816)

摘　　要：为比较参与大鼠声源定位功能的3个低位脑干核团主神经元的细胞形态及膜特性异同,用免疫荧光方法染色并观察3类脑干核团细胞形态异同。用低温切片的方法将SD大鼠脑干切成厚度为350μm的脑片,电流钳模式下记录各脑区主神经元的动作电位发放,并统计比较电生理特性参数。结果表明,红外光差显微镜及免疫荧光染色观察,3类神经元细胞胞体、树突及轴突差异大。在电生理记录过程中发现各神经元的放电模式表现各异。对电生理特性参数比较发现,3类神经元间的峰值幅度,去极化时程,复极化时程,第1、2动作电位间阈值,峰值时程差,第1动作电位延时均有显著统计学差异。3种低位脑干核团主神经元从形态特征到膜电生理特性的显著差异暗示了在听觉通路中3种主神经元通过对声信息不同的处理特性而确定声源定位的精准性。To compare the cell morphology and membrane characteristics of three primary brainstem nuclei with the function of source localization of rat,immunofluorescence staining was used to observe the morphological similarities and differences between the three types of brainstem nuclei.SD rat brain stem was cut into 350μm slices by low temperature slice method.The current clamp mode was used to record the action potential of the main neurons in the brain regions and theelectrophysiological parameters were statistically compared.The results show that three types of neuronal cell bodies,dendrites and axons have been observed by infrared differential microscope and immunofluorescence staining.During the electrophysiological recording,the discharge patterns of neurons under different stimulus intensities are different.Compared with electrophysiological parameters,the peak amplitude,depolarization time duration,repolarization duration,interspike interval at threshold and peak value,and first action potential delay of the three types of neurons are statistically significant differences.The significant differences of the membrane electrical properties and morphological characteristics of three lower brainstem nuclei of neurons in the auditory pathway suggests that the accuracy of the location of the sound source is determined by the different processing characteristics of the three kinds of primary neurons in the auditory pathway.

关 键 词：声源定位 梭形细胞 丛状细胞 内侧膝状体主细胞 动作电位 

分 类 号：Q424[生物学—神经生物学]