Characterization of Chiton Ischnochiton hakodadensis Foot Based on Transcriptome Sequencing  

作　　者：DOU Huaiqian MIAO Yan LI Yuli LI Yangping DAI Xiaoting ZHANG Xiaokang LIANG Pengyu LIU Weizhi WANG Shi BAO Zhenmin 

机构地区：[1]Key Laboratory of Marine Genetics and Breeding of Ministry of Education, Ocean University of China [2]Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology [3]Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology

出　　处：《Journal of Ocean University of China》2018年第3期632-640,共9页中国海洋大学学报（英文版）

基　　金：grant support from the National Natural Science Foundation of China (Nos. 31130054, 31472258);the AoShan Talents Program of Qingdao National Laboratory for Marine Science and Technology (No. 2015ASTP-ES02);the Fundamental Research Funds for the Central Universities (No. 201564009)

摘　　要：Chiton(Ischnochiton hakodadensis) is one of marine mollusks well known for its eight separate shell plates. I. hakodadensis is important, which plays a vital role in the ecosystems it inhabits. So far, the genetic studies on the chiton are scarce due in part to insufficient genomic resources available for this species. In this study, we investigated the transcriptome of the chiton foot using Illumina sequencing technology. The reads were assembled and clustered into 256461 unigenes, of which 42247 were divided into diverse functional categories by Gene Ontology(GO) annotation terms, and 17256 mapped onto 365 pathways by KEGG pathway mapping. Meanwhile, a set of differentially expressed genes(DEGs) between distal and proximal muscles were identified as the foot adhesive locomotion associated, thus were useful for our future studies. Moreover, up to 679384 high-quality single nucleotide polymorphisms(SNPs) and 19814 simple sequence repeats(SSRs) were identified in this study, which are valuable for subsequent studies on genetic diversity and variation. The transcriptomic resource obtained in this study should aid to future genetic and genomic studies of chiton.Chiton （Ischnochiton hakodadensis） is one of marine mollusks well known for its eight separate shell plates. I. hako-dadensis is important, which plays a vital role in the ecosystems it inhabits. So far, the genetic studies on the chiton are scarce due in part to insufficient genomic resources available for this species. In this study, we investigated the transcriptome of the chiton foot using Illumina sequencing technology. The reads were assembled and clustered into 256461 unigenes, of which 42247 were divided into diverse functional categories by Gene Ontology （GO） annotation terms, and 17256 mapped onto 365 pathways by KEGG path-way mapping. Meanwhile, a set of differentially expressed genes （DEGs） between distal and proximal muscles were identified as the foot adhesive locomotion associated, thus were useful for our future studies. Moreover, up to 679384 high-quality single nucleotide polymorphisms （SNPs） and 19814 simple sequence repeats （SSRs） were identified in this study, which are valuable for subsequent studies on genetic diversity and variation. The transcriptomic resource obtained in this study should aid to future genetic and genomic studies of chiton.

关 键 词：CHITON transcriptome sequencing DEG SNP SSR 

分 类 号：S917.4[农业科学—水产科学]