2012-2015年江苏省食源性肠炎沙门菌同源性分析  

Epidemiological characteristics and homologous analysis of Salmonella Enteritidis isolated in Jiangsu province from 2012 to 2015

作　　者：郑东宇[1] 马恺[1] 周翌婧[1] 唐震[1] ZHENG Dong-Yu;MA Kai;ZHOU Yi-Jing;TANG Zhen(Jiangsu Provincial Center For Disease Control and Prevention,Jiangsu Nanjing 210009, China)

机构地区：[1]江苏省疾病预防控制中心,江苏南京210009

出　　处：《江苏预防医学》2018年第3期256-258,共3页Jiangsu Journal of Preventive Medicine

基　　金：重大新发传染病综合防控科技示范工程(BE2015714)

摘　　要：目的了解江苏省食源性肠炎沙门菌的流行病学特征与PFGE分子分型。方法对2012—2015年来源于江苏省食品污染调查、食源性疾病监测及食源性疾病暴发事件中的肠炎沙门菌,开展PFGE分子分型并进行聚类分析。结果检测非伤寒沙门菌741株,其中肠炎沙门菌108株(14.6%),主要来源于食品与食源性疾病病例;2012年与2014年分离菌株最多,分别占总数的48.1%和26.9%;具有明显的季节性,主要为夏季(7—8月)、秋季(11—12月)两个高峰;108株肠炎沙门菌共有60个XbaⅠPFGE带型模式,14个克隆系及4个基因簇,P37为最主要的XbaⅠPFGE带型模式(n=18),M基因簇为最主要的独立基因簇(n=73,67.6%)。结论江苏省肠炎沙门菌的流行具有明显的季节性,在食品、病人均有分布;PFGE带型较多,但具有一个主要的基因簇,存在由单一带型的肠炎沙门菌引起暴发的风险。Objective To study the epidemiological characteristics and pulsed field gel electrophoresis（PFGE）molecular typing distribution of Salmonella Enteritidis isolated in Jiangsu province.Methods Salmonella Enteritidis isolated strains from the Food contamination investigation and the Surveillance of Foodborne diseases and the Outbreaks in Jiangsu province from 2012 to 2015 were typed by PFGE and subjected to cluster analysis.Results A total of 741 Salmonella typhi isolated strains were analyzed,among which 108 isolates（14.6%）were Salmonella Enteritidis,mainly from food and foodborne disease cases.The isolates in 2012 and 2014 accounted for 48.1% and 26.9% of all isolates.Obvious seasonal characteristics were observed,which were demonstrated as summer peak（Jul-Aug）and autumn peak（Nov-Dec）.The 108 Salmonella Enteritidis isolates generated 60 XbaⅠPFGE patterns in total,with 14 cloned lines and 4 genotypic clusters.P37 was dominant XbaⅠPFGE pattern（n=18）,M genotypic cluster was the major independent cluster.Conclusion The prevalence of Salmonella Enteritidis from 2012 to 2015 in Jiangsu province showed obvious seasonal characteristics,which distributed in food and patients.The PFGE patterns of Salmonella Enteritidis isolates were diverse.One of the main genetic clusters was identified,indicating outbreak risk by Salmonella Enteritidis of certain PFGE pattern.

关 键 词：肠炎沙门菌 PFGE 同源性分析 

分 类 号：R117[医药卫生—公共卫生与预防医学]