嵊州桃形李采后橘青霉分离鉴定及室内抑菌试验  

Isolation,Identification and Antibacterial Tests on Penicillium citrinumfor Postharvest Shengzhou Plums

作　　者：赵宁 宋昱辉 陈莉 莫亿伟[1] ZHAO Ning;SONG Yu hui;CHEN Li;MO Yi-wei(College of Life Science, Shaoxing University, Shaoxing, Zhejiang 312000, China;Shaoxing No. 1 High School, Shaoxing, Zhejiang 312000, China)

机构地区：[1]绍兴文理学院生命科学学院,浙江绍兴312000 [2]绍兴市第一中学,浙江绍兴312000

出　　处：《福建农业学报》2018年第4期407-412,共6页Fujian Journal of Agricultural Sciences

基　　金：浙江省绍兴市公益性技术应用研究计划项目(2017B70059);2013年嵊州市本级科技项目(No.64);公益性行业(农业)科研专项(201503142-13)

摘　　要：嵊州桃形李果实营养丰富,但成熟于高温季节,采后易受霉菌感染;为了减少霉菌对嵊州桃形李采后的感染,鉴定致病霉菌种类并开展抑菌试验很有必要。本研究从发病的嵊州桃形李果实分离到一株致病霉菌,经形态学和分子生物学鉴定,并用施保功和苯甲·嘧菌酯进行室内抑菌试验,结果发现:分离到的致病菌为橘青霉Penicillium citrinum(TXL-03),菌丝有分支且分生孢子串生;该菌株寄主具广谱性,对雪梨、苹果、油桃、樱桃、小番茄、葡萄、芒果、宝石李和香蕉接种后均可致病;施保功和苯甲·嘧菌酯均可有效抑制该菌丝生长和孢子萌发,但施保功的抑制效果更好,更适宜于嵊州桃形李采后霉菌防治。The renowned Shengzhou plums（Prunus salicina Lindl.var.taoxingli）ripen in the summer,and are high on nutritional value and appealing on eating quality.However,they turn soft rapidly and are prone to deterioration after harvesting in the summer heat.This study attempted to identify the source of microbial infection and determine an effective antibacterial measure to extend the shelf life for the prized fruits.Using the rDNA-ITS molecular identification and morphology methods,apathogen was isolated from the samples of diseased fruits.In the lab,the isolate was treated with Sporgon and difenoconazole＋azoxystrobin to confirm it to be Penicillium citrinum（TXL-03）.The fungus had numerous branches and conidiophores.It could infect a wide variety of fruits,such as pear,apple,nectarine,cherry,tomato,grape, mango,plum,and banana. Either Sporgon or difenoconazole＋azoxystrobin could retard the fungal growth.However,Sporgon appeared more effective,and thus,was recommended for treatment of the disease.

关 键 词：桃形李 橘青霉 分离鉴定 抑菌试验 

分 类 号：S482[农业科学—农药学]