难治性肺炎支原体肺炎患儿支气管肺泡灌洗液中肺表面活性物质相关蛋白的表达及其临床意义  

Clinical significance of the expression of pulmonary surfactant associated protein in bronchoalveolar lavage fluid of children with refractory Mycoplasma pneumoniae pneumonia

作　　者：原瑞芳 王少青 YUAN Rui-fang;WANG Shao-qing(Department of Pediatrics, the People＇s Hospital of Hebi City, Hebi 458030, Henan Province, Chin)

机构地区：[1]鹤壁市人民医院儿科,河南鹤壁458030

出　　处：《新乡医学院学报》2018年第6期528-530,共3页Journal of Xinxiang Medical University

摘　　要：目的探讨肺表面活性物质相关蛋白(SP)在难治性肺炎支原体肺炎(RMPP)患儿支气管肺泡灌洗液(BALF)中的表达及其临床意义。方法选择鹤壁市人民医院2015年1月至2016年12月收治的RMPP患儿30例,患儿分别于急性期和恢复期检测肺功能,并使用纤维支气管镜进行支气管肺泡灌洗,收集BALF,采用酶联免疫吸附试验检测BALF中SP-A、SP-B、SP-C和SP-D水平。结果 RMPP患儿急性期第1秒用力呼气量(FEV1)、用力肺活量(FVC)、FEV1/FVC分别为(1.34±0.23)L、(1.75±0.28)L、(68.25±6.21)%,恢复期FEV1、FVC、FEV1/FVC分别为(1.71±0.35)L、(1.98±0.36)L、(88.57±8.16)%;RMPP患儿恢复期FEV1、FVC、FEV1/FVC显著高于急性期(t=4.839、3.070、14.859,P<0.05)。RMPP患儿急性期BALF中SP-A、SP-B、SP-C及SP-D水平分别为(50.19±10.06)、(42.95±12.42)、(36.81±8.14)、(21.57±5.46)μg·L-1,恢复期BALF中SP-A、SP-B、SP-C及SP-D水平分别为(135.20±18.13)、(108.42±20.33)、(142.63±21.87)、(72.69±8.54)μg·L-1,RMPP患儿恢复期BALF中SP-A、SP-B、SP-C和SP-D水平显著高于急性期(t=22.457、15.052、24.837、27.623,P<0.05)。结论检测BALF中SP-A、SP-B、SP-C和SP-D水平对RMPP的诊断、病情评估、治疗及预后判断具有一定的指导意义。Objective To investigate the clinical significance of the expression of pulmonary surfactant associated protein（SP） in bronchoalveolar lavage fluid（BALF） of children with refractory Mycoplasma pneumoniae pneumonia（RMPP）.Methods Thirty children with RMPP were selected from January 2015 to December 2016 in the People＇ s Hospital of Hebi City. The lung function of the children was detected in acute and recovery stage,and bronchoalveolar lavage was performed with fiexible bronchofiberscope. The BALF was collected,and the levels of SP-A,SP-B,SP-C and SP-D in BALF were detected by enzyme linked immunosorbent assay. Results The forced expiratory volume in one second（FEV1）,forced vital capacity（FVC） and FEV1/FVC in RMPP children at acute stage were（1. 34 ± 0. 23） L,（1. 75 ± 0. 28） L and（68. 25 ± 6. 21） %respectively; and they were（1. 71 ± 0. 35） L,（1. 98 ± 0. 36） L and（88. 57 ± 8. 16） % respectively in the children at recovery stage. The FEV1,FVC and FEV1/FVC in RMPP children at recovery stage were significantly higher than those in the children at acute stage（t = 4. 839,3. 070,14. 859; P〈0. 05）. The levels of SP-A,SP-B,SP-C and SP-D in the RMPP children at acute stage were（50. 19 ± 10. 06）,（42. 95 ± 12. 42）,（36. 81 ± 8. 14） and（21. 57 ± 5. 46） μg·L-1 respectively; and they were（135. 20 ± 18. 13）,（108. 42 ± 20. 33）,（142. 63 ± 21. 87） and（72. 69 ± 8. 54） μg·L-1 respectively in the children at recovery stage. The levels of SP-A,SP-B,SP-C and SP-D in BALF of RMPP children at recovery stage were significantly higher than those in the children at acute stage（t = 22. 457,15. 052,24. 837,27. 623; P〈0. 05）. Conclusion The detection of SPA,SP-B,SP-C and SP-D levels in BALF plays a guiding role in the diagnosis,disease assessment,treatment and prognosis judgment of RMPP.

关 键 词：难治性肺炎支原体肺炎 肺表面活性蛋白 支气管肺泡灌洗液 

分 类 号：R375.2[医药卫生—病原生物学]