特发性矮小患儿IGFALS蛋白及IGFALS mRNA表达的变化  

Expression of IGFALS protein and IGFALS mRNA in children with idiopathic short stature

作　　者：王瑞琴 杨晓丽[2] 李静[2] WANG Ruiqin1, YANG Xiaoli2, LI Jing2(1. Department of Pediatrics, Shanxi Medical University, Taiyuan 030001, China; 2.Department of Pediatrics, Shanxi Provincial People＇s Hospita)

机构地区：[1]山西医科大学儿科医学系小儿内科教研室,太原030001 [2]山西省人民医院儿科

出　　处：《山西医科大学学报》2018年第3期277-279,共3页Journal of Shanxi Medical University

基　　金：山西省科技厅攻关基金资助项目(20140313016-3);安徽安科生物工程(集团)股份有限公司资助项目

摘　　要：目的检测特发性矮小患儿血清胰岛素样生长因子酸不稳定亚基(IGFALS)蛋白及IGFALS mRNA的水平。方法特发性矮小(idiopathic short stature,ISS)儿童26例,抽取10例正常身高儿童作为对照组。对其进行全面的病史采集和体格检查,并采用RT-PCR和ELISA法检测血清IGFALS mRNA及IGFALS蛋白表达水平。采用t检验、相关分析等统计学方法对结果进行分析。结果与对照组比较,ISS组血清IGFALS蛋白表达及IGFALS mRNA表达差异无统计学意义(P>0.05)。血清IGFALS蛋白及IGFALS mRNA与身高差均呈负相关(P<0.05);IGFALS蛋白与IGFALS mRNA表达呈正相关(P<0.05)。结论 IGFALS蛋白降低与ISS组患儿身高有关,可能是部分特发性矮小患儿的病因。Objective To investigate the levels of serum IGFALS protein and IGFALS mRNA in children with idiopathic short stature.Methods A total of 26 children with idiopathic short stature were admitted to this study as ISS group,and 10 age-matched normal height children were selected as control group. The comprehensive medical history and physical examination results were collected. Serum IGFALS mRNA expression and IGFALS protein level were measured by RT-PCR and ELISA way. The results were analyzed by statistical analysis of variance,t test and correlation analysis. Results The serum IGFALS protein expression and IGFALS mRNA expression showed no significant difference between ISS group and control group（ P〉0. 05）. The levels of IGFALS protein and IGFALS mRNA were both negatively correlated with height difference（ P〈0. 05）,and IGFALS protein expression was positively related to IGFALS mRNA expression（ P〈0. 05）. Conclusion The decrease of IGFALS protein is related to the height of ISS children,and it can be one of the etiology of children with idiopathic short stature.

关 键 词：特发性矮小 胰岛素样生长因子酸不稳定亚基 儿童 

分 类 号：R725[医药卫生—儿科]