Simultaneous Prediction of Retention Times and Peak Shapes of Sulfonamides in Reversed-Phase High-Performance Liquid Chromatography  

作　　者：Xiaobing Wei Zhengji Pang Guoliang Fan Xinhua Xu Lihong Wang 

机构地区：[1]School of Materials Science and Engineering,Tianjin University [2]Department of Applied Chemical Engineering,Ningbo Polytechnic [3]School of Agricultural and Food Engineering,Shandong University of Technology

出　　处：《Transactions of Tianjin University》2018年第3期256-262,共7页天津大学学报（英文版）

基　　金：supported by the National Nature Science Foundation of China (No. 51406109)

摘　　要：In this paper, we propose a novelmethod based on the plate theory to simultaneously predict retention times and peak shapes under gradient elutions and different flow rates by reversed-phase high-performance liquid chromatography. The proposed method yielded excellent retention prediction results in experiments with 16 common sulfonamides under 18 gradient conditions and four different flow rates, including 0.7, 1.0, 1.3, and 1.5 mL/min. The mean absolute deviation was 0.70%, which indicates accurate prediction. Moreover, the proposed method predicts the change wellin peak shapes caused by the expansion or compression ofpeaks under different gradient conditions.In this paper, we propose a novelmethod based on the plate theory to simultaneously predict retention times and peak shapes under gradient elutions and different flow rates by reversed-phase high-performance liquid chromatography. The proposed method yielded excellent retention prediction results in experiments with 16 common sulfonamides under 18 gradient conditions and four different flow rates, including 0.7, 1.0, 1.3, and 1.5 mL/min. The mean absolute deviation was 0.70%, which indicates accurate prediction. Moreover, the proposed method predicts the change wellin peak shapes caused by the expansion or compression ofpeaks under different gradient conditions.

关 键 词：Retention time Peak shape PREDICTION RP-HPLC SULFONAMIDE 

分 类 号：O657.72[理学—分析化学] TQ460.72[理学—化学]