桉树叶二种酶和蛋白质的季节与年际变化  

Dynamic Changes of Two Kinds of Enzymes and Soluble Protein of Eucalyptus Leaves in Different Seasons and Years

作　　者：朱宇林 周兴文 黄丽玲 苏建睦 赵英 ZHU Yu-lin;ZHOU Xing-wen;HUANG Li-ling;SU Jian-mu;ZHAO Ying(College of Biology and Pharmacy of Yulin Normal University,Yulin 537000, Guangxi,Chin)

机构地区：[1]玉林师范学院生物与制药学院,广西玉林537000

出　　处：《湖北农业科学》2018年第10期73-76,共4页Hubei Agricultural Sciences

基　　金：广西自然科学基金项目(2014GXNSFAA118083);广西大学生创新创业训练计划项目(201510606070)

摘　　要：桉树是中国南方地区的重要用材树种,探讨其贮藏氮素循环机制对于提高桉树人工林氮素利用水平具有一定的研究意义。试验以桉树枝叶组织为研究对象,在观察其物候期基础上,采用紫外分光度法测定了桉树叶蛋白质含量及谷氨酸合成酶和谷氨酰胺合成酶活性的动态变化。结果表明,秋季桉树叶中的可溶性蛋白含量最低,而在次年的春季其含量最高;夏季其含量则处在春季和秋季中间。谷氨酸合成酶活性在秋季为最高,在次年春季为最低。谷氨酰胺合成酶活性在秋季表现为最高,在次年的春季则表现为最低。桉树叶中的可溶性蛋白含量、谷氨酸合成酶活性以及谷氨酰胺合成酶活性与年份和季节密切相关,三者分别呈现出不同的变化趋势。Eucalyptus is an important economic tree species in southern China. Research on storage metabolism of eucalyptus would increase its nitrogen utilization level, which had certain research significance. Taking the branch and leaf tissue of eucalyptus as research object, the dynamic change of protein content as well as the activity of glutamate synthetase and glutamine synthetase was observed using UV spectrophotometric method based on the phonological phase. The results showed that, in the beginning of autumn, soluble protein content of eucalyptus leaves was at the lowest level; But in the following spring it was the highest. The soluble protein content in summer was between spring and the autumn. Glutamate synthese activity was the highest in autumn and the lowest in spring. The glutamine synthetase activity was the highest in autumn while lowest in spring. The glutamate synthetase activity, soluble protein content, glutamine synthetase activity was closely related to the season and year, the three of which present different changing trends.

关 键 词：桉树 可溶性蛋白 谷氨酰胺合成酶 谷氨酸合成酶 

分 类 号：S792.39[农业科学—林木遗传育种]