三羟基异黄酮对脂肪酸氧化关键酶的调控研究  

Regulatory Mechanism of Genistein on Key Enzymes of Fatty Acid Oxidation

作　　者：代子玲 范远景 杨登玲 徐柳 童金柱 娄鹏翔 DAI Zi-ling;FAN Yuan-jing;YANG Deng-ling(School of Food Science and Engineering,Hefei University of Technology, Hefei,Anhui 23600)

机构地区：[1]合肥工业大学食品科学与工程学院,安徽合肥236000

出　　处：《安徽农业科学》2018年第16期97-99,共3页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金项目(31071535)

摘　　要：[目的]探讨三羟基异黄酮(Genistein,Gen)对非酒精性脂肪肝病(NAFLD)模型中脂肪酸β氧化限速酶肉碱棕桐酰转移酶1(Carnitine acetyltransferase 1,CPT-1)的调节作用。[方法]油酸诱导细胞建模,实时荧光定量PCR测定三羟基异黄酮处理后细胞中过氧化物酶体增殖物激活受体α(PPARα)和CPT-1的mRNA相对表达量。[结果]Gen能经由PPARs途径上调CPT-1 mRNA的表达。[结论]该研究为三羟基异黄酮在临床药理上的应用以及非酒精性脂肪肝的预防治疗和药物研究提供了试验依据。[Objective]To investigate the regulatory mechanism of Genistein on CPT-1,a key rate-limiting enzyme of fatty acids in NAFLD model. [Method]The expression of mRNA PPARα and CPT-1 were measured by real-time fluorescence quantitative PCR. [Result]Gen can regulate the mRNA expression of CPT-1 through via PPARs pathway. [Conclusion]The study provides a test basis for the application of Genistein in clinical pharmacology and the prevention and treatment of NAFLD.

关 键 词：三羟基异黄酮 非酒精性脂肪肝病 过氧化物酶体增殖物激活受体Α 肉碱棕桐酰转移酶 

分 类 号：S188[农业科学—农业基础科学]