基于微流控技术的体外多参数快速诊断POCT系统的设计  

Multi-Parameter Point of Care Testing System Based on Micro-Nano Sensors and Microfluidics Technology

作　　者：王天星[1] 钟隆洁 苏凯麒[1] 秦臻[1] 胡宁[1] 王平[1] WANG Tianxing;ZHONG Longjie;SU Kaiqi;QIN Zhen;HU Ning;WANG Ping(Biosensor National Laboratory,Key Laboratory of Biomedical Engineering of Education Ministry,Department of Biomedical Engineering,Zhejiang University,Hangzhou 310027,China)

机构地区：[1]浙江大学生物传感器国家专业实验室,生物医学工程教育部重点实验室,生仪学院,杭州310027

出　　处：《传感技术学报》2018年第5期797-802,共6页Chinese Journal of Sensors and Actuators

基　　金：浙江省重大科技专项(2017C03032)

摘　　要：针对传统的体外快速诊断系统(POCT)精度低、参数单一等问题,设计了一种基于微纳传感结合微流控技术的多参数体外快速诊断POCT系统。本文研究了传感器与微流控芯片的制备工艺、传感器微弱信号的采集与提取、多通道参考电极的多维度抗干扰校正算法以及多参数POCT系统的优化设计,保证了POCT系统具有检测精度高、抗干扰性能好、多参数集成等特点。通过系统测试和临床实际样本的基本性能测试,实验结果表明,本文提出的多参数体外诊断快速检测POCT系统能够综合检测血糖、血酮等多项指标,具备快速,精确,多参数测量的特点,将为基层医疗机构的生化指标检测提供一种新型实用的技术和仪器。In order to solve the problems of low precision and single parameter in the traditional in-vitro rapid diagnostic system,a high precision and multi-parameter rapid in-vitro diagnostic system is designed based on the microfluidic and micro-nano sensing technology. The paper mainly includes the introduction and presentation of the following aspects： the microfluidic chip design,the micro-nano sensing preparation and processing technology,the multidimensional anti-interference correction algorithm based on microfluidic multi-channel reference technique,the weak biosensing signal extraction and acquisition,and the multi-parameter in-vitro rapid diagnostic analysis system design. The system has the advantages of high detection accuracy,good anti-jamming performance,and multi-parameter integration. The basic performance of the multi-parameter rapid detection system is tested using systematic test and clinical blood. The experimental results show that the multi-parameter in-vitro diagnostic rapid detection system can be a comprehensive detection of blood glucose,four items of blood lipids,uric acid,blood ketone and many other indicators,with fast accurate and multi-parameter measurement,which may provide a practical technology platform for the detection of portable biochemical indicators of basic medical institutions.

关 键 词：微纳传感技术 微流控 生物传感器 床旁检测 多参数分析系统 

分 类 号：R318[医药卫生—生物医学工程]