刺人参的化学成分研究  

Chemical Constituents from Oplopanax elatus

作　　者：邵莉[1] 王锦 罗伟[2] 黄卫华[2,3] 袁钧苏 SHAO Li;WANG Jin;LUO Wei;HUANG Wei-hua;YUAN Jun-su(Department of Pharmacognosy, School of Pharmacy, Hunan University of Chinese Medicne, Changsha 410208, China;Department of Clinical Pharmacology,Xiangya Hospital, Central South University, Changsha 410008, China;Tang Center for Herbal Medicine Research, University of Chicago, South Maryland Avenue 60637, USA)

机构地区：[1]湖南中医药大学药学院生药学教研室,湖南长沙410208 [2]中南大学湘雅医院临床药理研究所,湖南长沙410008 [3]美国芝加哥大学唐草药研究中心,芝加哥美国伊利诺伊州60637

出　　处：《中药材》2017年第10期2331-2334,共4页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金青年基金项目(31400306);中国博士后基金面上项目(2015M570692)

摘　　要：目的:研究刺人参的化学成分及其抗结肠癌活性。方法:采用各种色谱方法分离纯化刺人参活性部位的化学成分,根据理化性质和波谱数据鉴定结构。进行抗HCT-116和HT-29细胞增殖测试,研究其抗结肠癌活性。结果:从刺人参中分离并鉴定了11个化合物,其中5个炔醇:falcarinol(1)、falcarindiol(2)、oplopandiol(3)、oplopantriol A(4)、oplopantriol B(5);4个苯丙素:对甲氧基反式肉桂酸(6)、咖啡酸(7)、阿魏酸(8)、芥子酸(9);2个其他成分:(S,E)-nerolidol(10)、蔗糖(11)。刺人参提取物对人结肠癌细胞HCT-116和HT-29细胞的IC50分别为72.4±6.8μg/m L和96.3±7.5μg/m L。结论:其中,化合物1、4~10为首次从该植物中分离得到,化合物6和9首次从该属植物中分离得到。炔醇为刺人参抗结肠癌活性成分。Objective ： To investigate the chemical constituents and the anti-colorectal cancer activity of Oplopanax elatus. Methods ： The compounds from the active fraction were isolated and purified using chromatographic techniques, and identified by spectral data and the physicochemical properties. The extracts from Oplopanax elatus were screened for anti-proliferation on HCT-116 and HT-29 cancer cell lines. Results ： Eleven compounds had been purified and identified from the bioactive fractions including five polyynes ： falcarinol （ 1 ）, falcarindiol （ 2）, oplopandiol （ 3 ）, oplopantriol A （ 4）, oplopantriol B （ 5 ） ; four phenylpropanoids ： 4-methoxycinnamic acid （ 6 ）, caffeic acid（7） ,ferulic acid（8）and sinapic acid（9） ;the others： （S,E）-nerohdol（10） and sucrose（ 11 ）. The IC50 of the extract from the air-dried root of Oplopanax elatus on the anti-proliferation of HCT-116 and HT-29 cancer cell lines were 72.4±6. 8 μg/mL and 96. 3± 7.5 μg/mL, respectively. Conclusion ： Compounds 1,4- 10 are isolated from this plant for the first time, and compound 6,9 are firstly obtained from Oplopanax genus. Polyynes are responsible for the anti-eolorectal cancer activity of Oplopanax elatus.

关 键 词：刺人参 抗结肠癌 炔醇 苯丙素 

分 类 号：R284.1[医药卫生—中药学]