耐冬山茶基因组DNA的提取及CDDP引物的筛选  

Naidong Camellia(Camellia japonica) genomic DNA extraction and screening of CDDP primers

作　　者：隽逸豪 王绍霞 李春艳 马燕[1] 臧德奎[1] JUAN Yihao;WANG Shaoxia;LI Chunyan;MA Yan;ZANG Dekui(Forestry College, Shandong Agricultural University, Taian 271018;Shanting state-owned forest farm of Zaozhuang, Zaozhuang 277200;Forestry Bureau of Zaozhuang City Shanting District, Zaozhuang 277200)

机构地区：[1]山东农业大学林学院,山东泰安271018 [2]枣庄市山亭区国有山亭林场,山东枣庄277200 [3]枣庄市山亭区林业局,山东枣庄277200

出　　处：《山东林业科技》2018年第2期6-10,共5页Journal of Shandong Forestry Science and Technology

基　　金：山东省农业良种工程项目"林木种质资源收集保护与评价"[鲁农良字(2010)6号];特色优势及珍稀濒危林木种质资源收集保护(2016LZGC038)

摘　　要：根据山茶叶片次生代谢产物较多的特点,使用改良CTAB法提取耐冬山茶基因组DNA,所得基因组DNA纯度较高,满足分子实验要求。使用21条CDDP引物,在20μL包含2×Es Taq Master Mix(含染料)酶10μL、10 pmol·μL-1引物1μL、30 ng·μL-1DNA模板2μL及dd H2O 7μL的反应体系下对随机选择的样品进行扩增,得到11条多态性好,条带清晰的引物,为CDDP分子标记在山茶遗传多样性研究中的应用奠定了基础。According to the characteristics of the camellia leaves witch are rich in secondary metabolites, using the modified CTAB method to extract its genomic DNA.The purity of genomic DNA is high enough to meet the requirements of molecular experiments. Twenty-one CDDP primers are screened under the system with 10μL of 2×Es Taq Master Mix ferment（Containing Dyes）,1μL of 10 pmo L·μL-1 primer, 2μL of 30 ng·μL-1 DNA template and 7μL of dd H2 O. Eleven primers with rich polymorphism and clear bands were obtained. This study laid a foundation for the application of CDDP molecular markers in the study of genetic diversity of camellia.

关 键 词：山茶 改良CTAB法 CDDP 引物筛选 

分 类 号：Q781[生物学—分子生物学]