基于倏逝波界面散射的单个纳米颗粒无标记成像  

Single Nanoparticle Label-Free Imaging Based on Evanescent Wave In-Plane Scattering

作　　者：江丽雯 孙旭晴 刘虹遥[1] 谌雅琴[1] 熊伟[1] 张朝前[1] 路鑫超[1,3] Jiang Liwen;Sun Xuqing;Liu Hongyao;Chen Yaqin;Xiong Wei;Zhang Chaoqian;Lu Xinchao(Microelectronic Instrument and Equipment Center, Institute of Microelectronics Chinese Academy of Sciences, Beijing 100029, China;University of Chinese Academy of Sciences, Beijing 100049, China;Beijing Key Laboratory of IC Test Technology, Beijing 100088 China)

机构地区：[1]中国科学院微电子研究所微电子仪器设备研发中心,北京100029 [2]中国科学院大学,北京100049 [3]集成电路测试技术北京重点实验室,北京100088

出　　处：《光学学报》2018年第6期290-295,共6页Acta Optica Sinica

基　　金：国家自然科学基金(61302014;61404162;61205098);中国科学院科研装备研制项目(YZ201447);北京市自然科学基金(4162069);广东省科技计划项目(2014A040401053;2016A040403086);广州市科技计划项目(201604020005)

摘　　要：介绍了一种利用倏逝波界面散射对单个纳米颗粒进行无标记成像的方法。分别使用全内反射(TIR)倏逝波与表面等离激元(SPPs)两种倏逝波与单个纳米颗粒相互作用,激发纳米颗粒极化并发生散射,所产生的界面散射与入射倏逝波发生干涉,形成了纳米颗粒极化场与抛物线形干涉条纹的特征成像。分别对直径为500,200,100nm的聚苯乙烯颗粒进行了单个纳米颗粒无标记成像。比较了两种倏逝波界面散射对单个纳米颗粒成像结果,发现表面等离激元界面散射成像中的单个纳米颗粒极化强度约是全内反射极化强度的10倍,并且接近于暗场成像。因此,表面等离激元界面散射对单个纳米颗粒无标记成像具有更高灵敏度。所提单个纳米颗粒无标记成像方法可以拓展到病毒检测、生物单分子成像等领域。A method for label-free imaging to single nanoparticle by evanescent wave in-plane scattering is presented.Using the two evanescent waves of total internal reflection（TIR）evanescent wave and surface plasmon polaritons（SPPs）to interact with a single nanoparticle,respectively,which excites the nanoparticle polarization and scatter.Generated interfacial scattering is interfered with incident evanescent wave,which forms a characteristic imaging of the polarization field of the nanoparticles and the parabolic interference fringes.Single nanoparticle labelfree imaging is performed on three polystyrene nanoparticles with the diameters of 500,200,100 nm.The imaging results of two evanescent wave in-plane scattering on single nanoparticle are compared.It indicates that the nanoparticle polarization of SPPs in-plane scattering is approximately 10 times stronger than that of TIR in-plane scattering and close to dark field imaging.As a result,SPPs in-plane scattering manifests the better sensitivity to label-free single nanoparticle imaging.This single nanoparticle label-free imaging method can be extended to areas such as virus detection and single bio-molecule imaging.

关 键 词：表面光学 无标记成像 单个纳米颗粒 倏逝波界面散射 全内反射 表面等离激元 

分 类 号：O436.2[机械工程—光学工程]