不同品种猪不同泌乳期母乳外核体中miR-30a和miR-30d的差异表达研究  

Study on expression changes of the miR-30a and miR-30d in pig breast milk exosomes during different lactation periods

作　　者：梁艳[1] 顾以韧[1] 杨雪梅[1] 陶璇[1] 钟志君 杨跃奎[1] 曾凯[1] 陈晓晖[1] 吕学斌[1] LIANG Yan;GU Yiren;YANG Xuemei;TAO Xuan;ZHONG Zhijun;YANG Yuekui;ZENG Kai;CHEN Xiaohui;LV Xuebin(Animal Breeding and Genetics Key Laboratory of Sichuan Province, Sichuan Animal Science Academy, Chengdu 610066, China)

机构地区：[1]四川省畜牧科学研究院动物遗传育种四川省重点实验室

出　　处：《黑龙江畜牧兽医》2018年第11期106-108,112,共4页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家现代农业产业技术体系四川创新团队项目(sccxtd-001);国家生猪产业技术体系综合试验站项目(CARS-36);四川省畜牧科学研究院基本科研业务费专项(SASA2015A01);四川省科技支撑计划项目(2016NYZ0042);四川省科研院所科技成果转化项目(2017YSZH0014)

摘　　要：为了研究不同猪种不同泌乳期母乳外核体中miR-30a和miR-30d的表达模式,试验采用荧光定量PCR方法检测了不同泌乳期（0,3,7,14,21,28天）杜洛克猪和梅山猪母乳外核体中miR-30a和miR-30d的差异表达。结果表明：两品种猪母乳外核体中miR-30a和miR-30d的表达模式相似,均表现为0-3 d极显著高于7-28 d（P〈0.01）;梅山猪母乳外核体中miR-30a和miR-30d在0-3 d的表达量均高于杜洛克猪。说明miR-30a和miR-30d能够调控仔猪免疫系统发育。In order to study the expression patterns of miR-30 a and miR-30 d in pig breast milk exosomes during different lactation periods,the differential expression of miR-30 a and miR-30 d in breast milk exosomes of Duroc pig and Meishan pig was detected at different lactation periods（ 0,3,7,14,21 and 28 days） by fluorescence quantitative PCR（ Q-PCR） method. The results showed that the expression patterns of miR-30 a and miR-30 d in breast milk exosomes in two breeds were very similar across the entire lactation periods. In detail,the expression levels of miR-30 a and miR-30 d in 0-3 days were significantly higher than that in 7-28 days（ P〈0. 01）. The expression levels of miR-30 a and miR-30 d in Meishan pig were significantly higher than that in Duroc pig in 0-3 days（ P〈0. 01）. The results indicated that miR-30 a and miR-30 d could regulate the development of immune system in piglets.

关 键 词：猪 泌乳期 母乳 外核体 miR-30a miR-30d 

分 类 号：S828.3[农业科学—畜牧学]