急性呼吸道感染患儿中检出B1基因亚型人偏肺病毒  

B1 subgenotype of human metapneumovirus detected in children with acute respiratory infection

作　　者：范国权 刘晓莉[3] 蔚京京[2] Fan Gaoquan;Liu Xiaoli;Wei Jingjing(Department of Microbiology and Immunology, Basic Medical College of Shanxi Medical University, Taiyuan 030001 , China;Shanxi Children＇s Hospital, Taiyuan 030001 , Chin;Department of Pediatrics, Shanxi Medical University, Taiyuan 030001, China)

机构地区：[1]山西医科大学基础医学院微生物和免疫教研室,太原030001 [2]山西医科大学儿科医学系,太原030001 [3]山西省儿童医院,太原030013

出　　处：《中华实验和临床病毒学杂志》2018年第3期247-250,共4页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的初步了解急性呼吸道感染（acute respiratory infection, ARI）住院儿童中人偏肺病毒（human metapneumovirus, HMPV）感染情况。方法于2017年1月1日至12月31日在山西省儿童医院呼吸科采集居住在太原市的、年龄1～5岁的ARI儿童鼻咽洗液标本，通过逆转录-聚合酶链式扩增技术（reverse transcription-polymerase chain reaction, RT-PCR）检测HMPV核蛋白（nucleoprotein, N）基因，对扩增得到的阳性PCR产物进行序列测定、同源性比较和系统进化分析。结果于2017年2月份、11月份采集的标本中分别检测到1份、2份HMPV阳性标本。PCR产物核酸和推导氨基酸序列与GenBank中已有HMPV相应核酸和推导氨基酸序列同源性分别高于83%、90%。系统进化分析显示检测到的HMPV为B1基因亚型HMPV。结论太原市儿童ARI中流行的HMPV主要是B1基因亚型。Objective To reveal the prevalence of human metapneumovirus （HMPV） among pediatric patients with acute respiratory infection （ARI）. Methods Nasopharyngeal aspirates were collected from hospitalized young children （ 1-5 years） diagnosed as ARI in Department of Respiratory Disease, Shanxi Provincial Children＇s Hospital, Taiyuan city. The nucleoprotein （N） gene of HMPV was amplified by reverse transcription-polymerase chain reaction （RT-PCR）. The positive products of PCR were sequenced, made homology and phylogenetic analysis. Results One sample collected in Feb. 2017 and two samples collected in Nov. 2017 were HMPV positive in RT-PCR detection. The homologies of nucleie acid and deduced amino acid sequences of the three positive PCR products with the corresponding HMPV sequences in GenBank were over 83% and 90% , respectively. Phylogenetic analysis revealed that HMPV B1 subgenotype was detected. Conclusions HMPV B1 subgenotype was the major epidemic HMPV in young children with ARI in Taiyuan city.

关 键 词：人偏肺病毒 核蛋白 儿童 急性呼吸道感染 

分 类 号：R725.6[医药卫生—儿科]