潍坊地区儿童急性呼吸道感染病毒病原学分析  

Analysis of respiratory viruses in children with acute respiratory tract infection in Weifang

作　　者：蒋华芳[1] 李锦亮[2] 李丽[1] 王媛媛[1] 韩文宁[1] Jiang Huafang;Li Jinliang;Li Li;Wang Yuanyuan;Han Wenning(Department of Pediatrics, Department of Science and Education, Weifang Maternity and Children Healthcare Hospital, Weifang 261000, China)

机构地区：[1]潍坊市妇幼保健院儿科,261000 [2]潍坊市妇幼保健院科教科,261000

出　　处：《中华实验和临床病毒学杂志》2018年第3期272-276,共5页Chinese Journal of Experimental and Clinical Virology

基　　金：2016年潍坊市科学技术发展计划（医学类）（2016YX061）

摘　　要：目的了解潍坊地区急性呼吸道感染住院儿童病毒病原学情况。方法对2016年7月至2017年6月因急性呼吸道感染住院的患儿抽取鼻咽分泌物，采用基于毛细管电泳的多重RT-PCR法检测16种呼吸道病毒，包括甲型和乙型流感病毒（FluA、FluB）、季节性流感病毒H1N1亚型（sH1N1）、副流感病毒1、2、3型（PIV-1、2、3）、呼吸道合胞病毒A和B型（RSVA、RSVB）、人鼻病毒（HRV）、腺病毒（ADV）、人偏肺病毒（HMPV）、冠状病毒（CoV）NL63、229E、OC43、HKU1型以及人博卡病毒（BoV）。结果769例患儿中259例病毒检测阳性，总阳性率为33.68%（259/769），各病毒的阳性率依次为RSV（9.23%）、PIV（7.93%）、Flu（6.89%）、HRV（4.68%）、ADV（3.38%）、HMPV（1.69%）、CoV（0.91%）、BoV（0.65%）。病毒阳性率在各年龄组间差异有统计学意义（χ^2＝8.724, P＝0.033），以6个月～1岁组检出率最高。呼吸道病毒检出率在四季分布差异有统计学意义（χ^2＝16.103, P＝0.001），冬季检出率最高。结论RSV是潍坊地区儿童急性呼吸道感染的主要病毒，尤其在冬春季高发。呼吸道病毒的感染率与儿童年龄、发病季节等因素有关。ObjectiveTo explore the viral pathogens in hospitalized children with acute respiratory tract infection in Weifang.MethodsNasopharyngeal secretion （NPS） samples were collected from hospitalized patients with acute respiratory tract infection from July 2016 to June 2017. The NPS samples were detected for 16 respiratory virus types/subtypes including influenza A virus （FluA）, influenza B virus （FluB）, seasonal influenza A HlNl virus （sH1N1）, parainfluenza virus types 1, 2 and 3 （PIV-1, 2, 3）, respiratory syncytial virus A （RSVA）, respiratory syncytial virus B （RSVB）, human rhinovirus （HRV）, adenovirus （ADV）, human metapneumovirus （HMPV）, four coronavirus sybtypes （Cov-NL63, 229E, OC43, HKUl） and human bocavirus （BoV） by multiplex reverse transcription polymerase chain reaction （RT-PCR） assays based on automatic capillary electrophoresis.ResultsA total of 769 children with respiratory tract infection were enrolled, and the overall positive rate for the 16 common respiratory tract viruses was 33.68% （259/769）. The positive rates were as follows： RSV （9.23%）, PIV （7.93%）, Flu （6.89%）, HRV （4.68%）, ADV （3.38%）, HMPV （1.69%）, CoV （0.91%）, BoV （0.65%）. The positive rate of viral detection showed significant differences among different age groups （χ^2=8.724, P=0.033）, and the highest positive rate was noted in the age group of 6 months to 1 year. The overall positive rate of viral detection showed a significant difference in terms of seasonal distribution, with a peak prevalence in winter.ConclusionsRSV was the main respiratory tract virus among children in Weifang, especially in winter and spring. The distribution of viruses in children with respiratory tract infection was associated with age and season.

关 键 词：急性呼吸道感染 呼口发道病毒 儿童 

分 类 号：R725.6[医药卫生—儿科]