PTH-FC对大鼠骨髓间充质干细胞增殖及骨向分化的影响  

Effects of PTH-FC on Proliferation and Osteogenic Differentiation of Rat Bone Marrow Mesenchymal Stem Cells

作　　者：刘艳云 徐宁 刘玉慧[1] 刘加涛 朱艺丹 何苗苗[1] 余占海[1] LIU Yan-yun;XU Ning;LIU Yu-hui;LIU Jia-tao;ZHU Yi-dan;HE Miao-miao;YU Zhan-hai(Department of Implantology , School and Hospital of Stomatology , Lanzhou University, Lanzhou 730000, Chin)

机构地区：[1]兰州大学口腔医学院(医院)种植科,甘肃兰州730000

出　　处：《口腔医学研究》2018年第6期614-618,共5页Journal of Oral Science Research

基　　金：兰州市人才创新创业项目(编号:2014-RC-79)

摘　　要：目的:评价甲状旁腺激素融合蛋白PTH-FC(parathyroid hormone-Fc)对大鼠骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)骨向分化的作用,筛选出PTH-FC诱导BMSCs最佳的作用时间。方法:采用全骨髓贴壁法分离培养大鼠BMSCs并进行鉴定,以1.0×10^(-9) mol/L PTH-FC成骨诱导液诱导BMSCs作用6h(间歇性给药组)和48h(持续性给药组)通过噻唑蓝(methylthiazolyldiphenyl-tetrazolium bromide,MTT)、碱性磷酸酶(alkaline phosphatase,ALP)、茜素红染色观察PTH-FC对BMSCs增殖和骨向分化的作用。结果:含1.0×10^(-9) mol/L PTH-FC的成骨培养液作用6h间歇性给药组与其他组比较,ALP和茜素红染色结果显著高于其他组;MTT结果显示各实验组间比较差异无统计学意义。结论:1.0×10^(-9) mol/L PTH-FC作用BMSCs 6h的间歇性给药组具有最佳的骨向诱导能力,这提示PTH-FC作为骨诱导生长因子联合BMSCs对骨组织缺损的修复是可行的。Objective：To evaluate the effects of PTH-FC on osteogenic differentiation of rat bone marrow mesenchymal stem cells（BMSCs）,and to screen out the optimal time of BMSCs induced by PTH-FC.Methods：BMSCs from rats were isolated and cultured in vitro using the whole bone marrow adherence method and identified.BMSCs was induced with PTH-FC（10^-9 mol/L）osteogenic inducer for 6 h（intermittent administration）and 48 h（continuous administration）.The best proliferation promoting and osteogenic differentiation promoting effect of PTH-FC on BMSCs was evaluated by means of MTT,ALP,and alizarin red staining.Results：ALP and alizarin red staining in the osteogenic culture medium of PTH-FC（10^-9 mol/L）which acted on BMSCs for 6 hwere significantly higher than those of other groups（P〈0.05）,however,the cell proliferation result was not significant different between the experimental groups.Conclusion：The combination of PTH-FC,which is used as a bone induced growth factor,with BMSCs to repair bone tissue defects is feasible.

关 键 词：甲状旁腺激素融合蛋白PTH-FC 骨髓间充质干细胞 骨向分化 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]