Lgr5阳性干细胞在3D培养中形成类器官的研究进展  

Research progress of Lgr5-positive stem ceils in the formation of organoid in 3D culture

作　　者：何庆庆 黎奥 王茂华 高下[1] He Qingqing;Li Ao;Wang Maohua;Gao Xia(Department of Otorhinolaryngology Head and Neck Surgery, Affiliated Drum Tower Hospital of Nanjing University Medical School, Research Institute of Otolaryngology, 210008 Nanjing, China;Department of Otorhinolaryngology Head and Neck Surgery, Xiangya No. 2 Hospital of Central South University, 410011 Changsha , China)

机构地区：[1]南京大学医学院附属鼓楼医院耳鼻咽喉头颈外科,耳鼻咽喉研究所,南京210008 [2]中南大学湘雅二医院耳鼻咽喉头颈外科,长沙410011

出　　处：《中华耳鼻咽喉头颈外科杂志》2018年第6期469-473,共5页Chinese Journal of Otorhinolaryngology Head and Neck Surgery

基　　金：中央高校基本科研业务费专项资金（021414380311）;江苏省科教强卫工程项目医学重点学科（ZDXKB2016015）;南京市卫计委科技发展资金一般性课题项目（YKK17061）

摘　　要：干细胞是人体中组织器官再生的基石,如何充分利用其多能性促进机体细胞、组织和器官的修复或再生是生物医学研究中长久以来的热点和重点.Lgr5阳性细胞是机体中具有多能性分化能力的干细胞样细胞之一,存在于小鼠或人类的多种器官或组织中.目前研究表明,单个或少量的Lgr5阳性干细胞可以在3D培养体系中生长形成多种类器官（organoid）,这些类器官的某些生物学及生理学特性与其来源的器官类似或一致.这些实验拓宽了研究者的视野,同时也开辟了一种新的干细胞研究方向.对于内耳细胞再生研究来说,Lgr5阳性内耳干细胞正是近年来刚被证实的内耳多能性细胞群.如何从来源于Lgr5阳性干细胞的类器官研究中获得灵感或经验,对于内耳干细胞的研究具有前瞻性指导意义.本文将对Lgr5阳性干细胞在3D培养体系中形成类器官的相关研究进行综述,以期为类内耳器官的形成与应用提供新思路.Stem cell is critical to regeneration of tissue or organ of human.How to promote repair or regeneration in the tissues/organ using its pluripotency is always an important issue.Lgr5-possitive cell is one type of the stem cell-like cells capable of pluripotent differentiation in various tissues/organs of both hunans and mice.Current study showed that single or small amount Lgr5-possitive stem cells can grow and form a plurality of organs in 3D culture system,and some organs can present similar biological and physiological properties with the progenitor they were derived.These studies provided new insight into future orientation,for example,Lgr5-possitive inner ear cells were confirmed as inner ear pluripotent cells population,the experiences obtained from organoid studies of Lgr5-possitive cells have certainly showed potential in the future study of inner ear stem cells.This review will focus on the recent progress associated with Lgr 5-positive stem cells forming organoids in the 3D culture.

关 键 词：干细胞 类器官 器官培养技术 再生 

分 类 号：R318[医药卫生—生物医学工程]