葛根素药物纳米晶自稳定pickering乳的大鼠肠吸收特性研究  

Intestinal absorption of puerarin nanocrystalline self-stabilizing pickering emulsion in rats

作　　者：王帅[1] 李清清 王帆[1] 易涛[2] 张继芬[1] WANG Shuai;LI Qing-qing;WANG Fan;YI Tao;ZHANG Ji-fen(College of Pharmaceutical Sciences, Southwest Univrsity, Chongqing 400716, China;School of Health Sciences, Macao Polytechnic Institute, Macao 999078, China)

机构地区：[1]西南大学药学院,重庆400716 [2]澳门理工学院高等卫生学校,澳门999078

出　　处：《中国中药杂志》2018年第10期2162-2167,共6页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81603304);重庆市卫生局中医药科技重点项目(ZY201701004);澳门特别行政区科学技术发展基金项目(001/2016/A1)

摘　　要：研究药物纳米晶自稳定pickering乳（NSSPE）的肠吸收性质。采用大鼠在体单向肠灌流模型,高效液相色谱法测定灌流液中葛根素的浓度,考察NSSPE在十二指肠、空肠、回肠和结肠中的吸收速率常数（Ka）和表观渗透系数（Papp）,并与葛根素的原料药、纳米晶和普通乳进行比较。结果显示,葛根素NSSPE在各肠段Ka和Papp大小依次为：十二指肠〉空肠〉回肠〉结肠。其中十二指肠显著大于空肠和回肠（P〈0.05）,极显著大于结肠（P〈0.01）,空肠和回肠间没有显著性差异。与其余3种制剂比较,葛根素NSSPE在十二指肠中的Ka和Papp显著大于原料药、纳米晶和普通乳（P〈0.05）;空肠和结肠中的Ka和Papp极显著大于原料药、纳米晶和普通乳（P〈0.01）;回肠中的Ka和Papp显著大于葛根素的原料药和普通乳（P〈0.05）,但与纳米晶无显著差别。由此证实,NSSPE能显著提高葛根素在小肠中的吸收。To study the intestinal absorption characteristics of drug’s nanocrystalline self-stabilizing Pickering emulsion（ NSSPE） in situ in rats. Rat single-pass intestinal perfusion model was established,and high performance liquid chromatography（ HPLC） was used to detect the concentration of puerarin in rat intestinal perfusion solution,assay the absorption rate constant（ Ka） and the intestinal apparent permeability coefficient（ Papp） of NSSPE in duodenum,jejunum,ileum,and colon,which were compared with those of raw material,nanocrystal and normal emulsion,respectively. For NSSPE,the Kaand Pappvalues were in the following order： duodenum 〉jejunum 〉 ileum（ P 〈 0. 05） 〉 colon（ P 〈 0. 01）. However,there was no obvious difference between jejunum and ileum. As compared with raw material,nanocrystal and normal emulsion,the Kaand Pappvalues of NSSPE in duodenum were significantly higher than those of other three preparations（ P 〈 0. 05）; and the Kaand Pappvalues of NSSPE in jejunum and colon were significantly higher than those of raw material,nanocrystal and normal emulsion（ P 〈 0. 01）; and the Kaand Pappof NSSPE in ileum were also higher than those of raw material and normal emulsion（ P 〈 0. 05）,but had no obvious difference with nanocrystal. The results showed that NSSPE could significantly improve the absorption of puerarin in the intestine of rats.

关 键 词：葛根素 纳米晶 pickering乳液 单向肠灌流 肠吸收 

分 类 号：R285.5[医药卫生—中药学]