Plant G proteins interact with endoplasmic reticulum luminal protein receptors to regulate endoplasmic reticulum retrieval  

作　　者：Shanshan Wang Ke Xie Guoyong Xu Huarui Zhou Qiang Guo Jingyi Wu Zengwei Liao Na Liu Yan Wang Yule Liu 

机构地区：[1]MOE Key Laboratory of Bioinformatics, Center for Plant Biology, Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing 100084, China [2]Advanced Biotechnology and Application Research Center, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, China [3]The Institute for Advanced Studies (IAS), Wuhan University, Wuhan 430072, China

出　　处：《Journal of Integrative Plant Biology》2018年第7期541-561,共21页植物学报（英文版）

基　　金：supported by the National Basic Research Program of China (2017YFA0503401);the National Natural Science Foundation of China (31530059, 3142100007, 31470254);the National Transgenic Program of China (2016ZX08009-003, 2016ZX08005-001 and 2016ZX08009001-004)

摘　　要：Maintaining endoplasmic reticulum （ER） homeostasis is essential for the production of biomolecules. ER retrieval, i.e., the retrograde transport of compounds from the Golgi to the ER, is one of the pathways that ensures ER homeostasis. However, the mechanisms underlying the regulation of ER retrieval in plants remain largely unknown. Plant ERD2-1ike proteins （ERD2s） were recently suggested to function as ER luminal protein receptors that mediate ER retrieval. Here, we demon- strate that heterotrimeric G protein signaling is involved in ERD2-mediated ER retrieval. We show that ERD2s interact with the heterotrimeric G protein Gα and Gγsubunits at the Golgi. Silencing of Gα, Gβ, or Gγ increased the retention of ER luminal proteins. Furthermore, overexpression of Gα, Gβ, or Gγ caused ER luminal proteins to escape from the ER, as did the co-silencing of ERD2a and ERD2b. These results suggest that G proteins interact with ER luminal protein receptors to regulate ER retrieval.Maintaining endoplasmic reticulum （ER） homeostasis is essential for the production of biomolecules. ER retrieval, i.e., the retrograde transport of compounds from the Golgi to the ER, is one of the pathways that ensures ER homeostasis. However, the mechanisms underlying the regulation of ER retrieval in plants remain largely unknown. Plant ERD2-1ike proteins （ERD2s） were recently suggested to function as ER luminal protein receptors that mediate ER retrieval. Here, we demon- strate that heterotrimeric G protein signaling is involved in ERD2-mediated ER retrieval. We show that ERD2s interact with the heterotrimeric G protein Gα and Gγsubunits at the Golgi. Silencing of Gα, Gβ, or Gγ increased the retention of ER luminal proteins. Furthermore, overexpression of Gα, Gβ, or Gγ caused ER luminal proteins to escape from the ER, as did the co-silencing of ERD2a and ERD2b. These results suggest that G proteins interact with ER luminal protein receptors to regulate ER retrieval.

关 键 词：Chun-Ming Liu Institute of Crop Science CAAS Institute of Botany CAS China 

分 类 号：Q945[生物学—植物学]