过量硒加重二硝基氯苯诱导的小鼠特应性皮炎样表现  

Excessive selenium supplementation aggravates atopic dermatitis-like skin lesions induced bydinitrochlorobenzene in mice

作　　者：莫俊銮[1] 张丽君[1] 周继昌[1] 龚春梅[1] 徐远飞[1] 杨慧[1] Mo Junluan;Zhang Lijun;Zhou Jichang;Gong Chunmei;Xu Yuanfei;Yang Hui(Molecular Biology Laboratory, Shenzhen Center for Chronic Disease Control, Shenzhen 518020, China)

机构地区：[1]深圳市慢性病防治中心分子生物学实验室,518020

出　　处：《中华皮肤科杂志》2018年第7期495-499,共5页Chinese Journal of Dermatology

基　　金：国家自然科学基金（81172669）;深圳市科技计划项目（JcYJ20150402151501943）;深圳市卫生计生系统科研项目（201401069）

摘　　要：目的 观察硒对小鼠特应性皮炎样表现的影响。方法 40只BALB/c小鼠随机等分为缺硒组（0.01 mg/kg）、正常硒组（0.25 mg/kg）、过量硒组（3.00 mg/kg）和对照组（0.25 mg/kg），4组小鼠先用缺硒饲料饲养4周后，再分别以缺硒、正常硒、过量硒和正常硒饲料喂养4周。此后，3个致敏组（缺硒组、正常硒组、过量硒组）采用二硝基氯苯（DNCB）诱导特应性皮炎样表现。激发期间监测小鼠皮炎表现严重程度。激发3周后检测小鼠血浆总IgE、全血炎症细胞计数，并剪取小鼠背部皮肤组织进行组织病理检查，分析组织中硒含量。单因素方差分析方法评估多组间IgE、硒水平和细胞计数等检测结果差异, Pearson相关性分析和线性回归分析评估IgE等检测指标与硒水平的相关性。结果 致敏6 d后，致敏组小鼠皮肤表现评分明显高于对照组（致敏第6、8、11、13、15、18天时各组间比较，F值分别为44.897、76.622、114.866、33.352、28.605、11.271，均P 〈 0.01），第11天过量硒组皮肤表现评分明显高于缺硒组和正常硒组（均P 〈 0.05）。与对照组相比，致敏小鼠均发生明显的皮炎病理改变，但3个致敏组之间皮损中炎症细胞计数差异无统计学意义（均P 〉 0.05）。致敏小鼠全血炎症细胞和血浆总IgE水平均随膳食硒水平的增加而升高；其中过量硒组血浆总IgE水平升高最明显，与缺硒组、正常硒组和对照组比较，差异均有统计学意义［（167.17 ± 8.49） μg/L比（124.78 ± 5.32） μg/L、（132.61 ± 4.71） μg/L、（109.13 ± 0.79） μg/L，t值分别为3.919、3.222、6.485，均P 〈 0.05］。致敏组小鼠皮肤组织硒含量与小鼠血浆总IgE水平（r = 0.579，P 〈 0.001）、全血白细胞（r = 0.414，P 〈 0.05）、中性粒细胞（r = 0.439，P 〈 0.05）、淋巴细胞（r = 0.417，P 〈 0.05）和嗜酸性粒细胞（r = 0.505，P 〈 0.01）均呈线性正相关。结�Objective To evaluate the effect of micronutrient selenium on atopic dermatitis-like skin lesions in mice. Methods After 4-week feed with forages lacking selenium, 40 BALB/c mice were randomly and equally divided into 4 groups： selenium deficiency group fed with forages containing 0.01 mg/kg selenium for 4 weeks, normal selenium supplementation group fed with forages containing 0.25 mg/kg selenium for 4 weeks, excessive selenium supplementation group fed with forages containing 3.00 mg/kg selenium for 4 weeks, and control group fed with forages containing 0.25 mg/kg selenium for 4 weeks. Then, atopic dermatitis-like skin lesions were induced by dinitrochlorobenzene （DNCB） in the mice in sensitized groups, including the selenium deficiency group, normal selenium supplementation group and excessive selenium supplementation group. During sensitization, the severity of dermatitis in mice was monitored. Three weeks after the sensitization, the total plasma IgE level and inflammatory cell count in whole blood were measured. Skin tissues from the back of mice were subjected to histopathological examination and the selenium level was detected. Statistical analysis was carried out by one-way analysis of variance for comparisons of IgE levels and cell counts among different groups, as well as by Pearson correlation analysis and linear regression analysis for analyzing the correlation of various indices with the selenium level. Results Six days after the sensitization, the dermatitis severity scores were significantly higher in the sensitized groups than in the control group （On day 6, 8, 11, 13, 15 and 18 after the sensitization, F = 44.897, 76.622, 114.866, 33.352, 28.605 and 11.271 respectively, all P 〈 0.01）. On day 11 after the sensitization, the dermatitis severity score was significantly higher in the excessive selenium supplementation group than in the selenium deficiency group and normal selenium supplementation group （both P 〈 0.05）. Compared with the control group, obvious pathological changes o

关 键 词：皮炎 特应性 硒 疾病模型 动物 炎症 免疫球蛋白E 

分 类 号：R758.2[医药卫生—皮肤病学与性病学]