柴胡皂甙-D通过调节SIRT3对MPP+诱导的SH-SY5Y细胞模型的神经保护作用  

Neuroprotective Effects of Saikosaponin-d on MPP+-induced Cytotoxicity in SH-SY5Y cells via Regulating SIRT3

作　　者：杨军 张贵斌 陈冬娟 YANG Jun;ZHANG Gui-bin;CHEN Dong-juan(Department of Neurology, Xiangyang No. 1 People＇s Hospital, Hubei University of Medicine, Xiangyang, Hubei 441000 China)

机构地区：[1]湖北医药学院附属襄阳市第一人民医院神经内科,湖北襄阳441000

出　　处：《湖北医药学院学报》2018年第1期29-34,共6页Journal of Hubei University of Medicine

基　　金：湖北省卫计委面上项目(WJ2017M231)

摘　　要：目的:研究柴胡皂甙d(Ssd)对帕金森病细胞模型的神经保护作用。方法:利用不同浓度的Ssd培育MPP+诱导的SH-SY5Y细胞模型,观察柴胡皂甙d对帕金森病细胞模型的神经保护作用。使用基因敲除的方法,进一步研究其潜在的作用机制。结果:Ssd对MPP+诱导的SH-SY5Y细胞模型具有神经保护作用,且呈浓度依赖性。MPP+作用会导致氧化应激增强,凋亡细胞增多,caspase-3的活性增强。用Ssd处理后这些效应会减弱。进一步研究发现,Ssd显著增加SIRT3的mRNA和蛋白表达。而在敲除SIRT3后,Ssd作用于SH-SY5Y细胞的保护机制彻底消除。结论:Ssd对MPP+诱导的SH-SY5Y细胞模型具有神经保护作用,该作用是通过上调SIRT3表达实现的。Objective To study the neuroprotective effects of saikosaponin-d（ Ssd） on the cell model of Parkinson＇s disease.Methods The MPP-induced SH-SY5Y cell model was cultured with different concentrations of Ssd to observe the neuroprotective effect of Ssd on Parkinson＇s disease cell model. The gene knockout method was used to further study the potential mechanism.Results Our study showed that Ssd exerted a concentration-dependent neuroprotective effect against MPP ＋-induced SH-SY5Y cell model of Parkinson＇s disease. In addition,MPP ＋ exposure resulted in enhanced oxidative stress,increased apoptotic cell death and enhanced caspase-3 activity. These effects were attenuated after Ssd treatment. Further study found that Ssd significantly increased SIRT3 mRNA and protein expression. After knockout of SIRT3,the protective mechanism of Ssd on SH-SY5Y cells was completely eliminated. Conclusion Ssd has a neuroprotective effect on MPP ＋-induced SH-SY5Y cell model by up-regulating the expression of SIRT3.

关 键 词：柴胡皂甙D 帕金森病 氧化应激 SIRT3 

分 类 号：R285[医药卫生—中药学]