金银花种质资源DNA身份证构建及遗传相似性分析  

Establishment of DNA identity card and analysis of genetic similarity among 58 varieties in Lonicera japonica

作　　者：朱凤洁 张山山[2] 袁媛[2] 周骏辉[2] 蒋超[2] 杨健[2] 刘娟[2] 俞年军 黄璐琦[2] ZHU Feng-jie;ZHANG Shan-shan;YUAN Yuan;ZHOU Jun-hui;JIANG Chao;YANG Jian;LIU Juan;YU Nian-jun;HUANG Lu-qi(School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China;National Resource Center for Chinese Meteria Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China)

机构地区：[1]安徽中医药大学药学院,安徽合肥230012 [2]中国中医科学院中药资源中心,北京100700

出　　处：《中国中药杂志》2018年第9期1825-1831,共7页China Journal of Chinese Materia Medica

基　　金：科技基础性工作专项(2015FY111500);中央级公益性科研院所基本科研业务费专项资金项目(ZZ10-008)

摘　　要：利用22对SSR引物对20个产地58个金银花农家种进行扩增,从中筛选出7对多态性较强、扩增带型稳定的引物建立金银花种质资源DNA身份证。结果表明,7对核心引物可将58个金银花农家种区分。采用Pop Gene32（vesion1.32）软件包对58个金银花农家种进行遗传相似性分析,相似系数变异范围为0.366 7~0.916 7。依据相似系数,可反映58个金银花农家种之间的亲缘关系,并将58个农家种遗传一致性分为4类,为金银花的优良种质选育提供参考信息,为中药材道地性研究提供理论依据。A total of 58 varieties in Lonicera japonica from 20 producing areas were amplified by 22 pairs of SSR primers. Seven pairs of polymorphic primers were screened and their primers were used to establish DNA identity card and analyze genetic similarity.All the 58 varieties could be distinguished each other by the DNA identity card constituted by 7 pairs of core SSR primers. The genetic similarity coefficients of 58 varieties ranged from 0. 366 7 to 0. 916 7 by using Pop Gene32（ vesion1. 32）. Furthermore,all the varieties consistency were classified into 4 groups and constructed an evaluation table according to cluster analysis by an un-weighted pair-group average method with arithmetic mean. As expected,the results of cluster and evaluation table reflected 58 varieties relatives,which provide reference information for the selection of fine germplasm of L. japonica and the theoretical basis for the study of Dao-di herbs.

关 键 词：金银花 DNA身份证 遗传相似系数 

分 类 号：S567.79[农业科学—中草药栽培]