甘肃红芪中黄芪甲苷存在的判定以及一年生、二年生红芪、黄芪皂苷和总黄酮含量对比  

Determination of Astragaloside Ⅳ in Gansu Hedysari Radix and Comparison of Astragaloside Ⅳ and Total Saponins Content in One Year Old and Two Years Old Hedysari Radix and Astragali Radix

作　　者：叶迎[1] 包强[2] 王瑞海[1] 柏冬[1] 田生华 刘培元 刘卫东 彭建平 陈永刚 杨丽霞[2] 姜良恩 刘丽梅[1] YE Ying;BAO Qiang;WANG Rui-hai;BAI Dong;TIAN Sheng-hua;LIU Pei-yuan;LIU Wei-dong;PENG Jian-ping;CHEN Yong-gang;YANG Li-xia;JIANG Liang-en;LIU Li-mei(Institute of Basic Theory Research of Traditional Chinese Medicine （TCM） , China Academy of Chinese Medical Sciences, Beijing 100700, China;Gansu Province Hospital of TCM, Lanzhou 730050, China;Chinese Medicinal Materials Center of Longnan City Wudu County of Gansu Province, Longnan 746000, China;Chinese Herbal Medicine Industry Development Center of Tanchang County of Gansu Province, Longnan 748500, China)

机构地区：[1]中国中医科学院中医基础理论研究所,北京100700 [2]甘肃省中医院,兰州730050 [3]甘肃省陇南市武都区中药材中心,甘肃陇南746000 [4]甘肃省宕昌县中药材产业开发中心,甘肃陇南748500

出　　处：《中国实验方剂学杂志》2018年第14期69-75,共7页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：中国中医科学院自主选题(YZ-1438)

摘　　要：目的：判定甘肃红芪是否含有黄芪甲苷;测定甘肃不同产地二年生红芪和黄芪总黄酮总皂苷含量,对比分析一年生、二年生红芪和黄芪总黄酮总皂苷含量。方法：以黄芪甲苷为对照品,采用HPLC-MS联用法检测红芪和黄芪中黄芪甲苷的含量;以毛蕊异黄酮葡萄糖苷为对照品,检测波长为260nm,采用紫外分光光度法测定红芪和黄芪中总黄酮的含量;以黄芪甲苷为对照品,采用香草醛-高氯酸显色法,检测波长为540nm,可见分光光度法测总皂苷的含量。结果：测定样品中,甘肃红芪中黄芪甲苷的质量分数为0.0555μg·g^-1,黄芪中黄芪甲苷的质量分数为37.9μg·g^-1;二年生红芪总黄酮质量分数,陇西等地为0.449%,宕昌为0.370%,武都为0.394%,全省平均为0.402%;黄芪总黄酮质量分数,陇西等地为0.374%,宕昌为0.542%,武都为0.424%,全省平均为0.452%;二年生红芪总皂苷质量分数,陇西等地为3.68%,宕昌为3.89%,武都为4.24%,全省平均为3.95%,黄芪总皂苷质量分数,陇西等地为4.63%,宕昌为5.11%,武都为4.80%,全省平均为4.86%。结论：甘肃红芪中含有黄芪甲苷,红芪含有的黄芪甲苷仅为黄芪的1/682;甘肃不同产地一年生、二年生红芪总黄酮、总皂苷含量均低于黄芪。二年生红芪和黄芪中总黄酮、总皂苷含量均高于一年生。一年生、二年生红芪中总黄酮、总皂苷含量均为武都优于宕昌,一年生、二年生黄芪总黄酮、总皂苷含量均为宕昌优于武都。红芪为武都地区二年生质量最优,黄芪为宕昌地区二年生质量最优。黄芪甲苷、总皂苷、总黄酮含量差异明显,二者不适合替代使用。Objective： To determine whether Gansu Hedysari Radix contains astragaloside Ⅳ; to determinate the content of the total flavonoids and the total saponins in Hedysari Radix and Astragali Radix,and compare the total flavonoids and total saponins content of one year old and two years old Hedysari Radix and Astragali Radix in different areas of Gansu province. Method： The content of astragaloside Ⅳ was determined by high-performance liquid chromatography-mass spectrometry（ HPLC-MS） with astragaloside Ⅳ as the reference substance. The content of total flavonoids was determined by ultraviolet spectrophotometry at 260 nm detection wavelength with myroneisoflavones glucoside as the reference substance. The content of total saponins was determined by vanillin-perchloric acid colorimetric method at 540 nm wavelength with astragaloside Ⅳ as the reference substance. Result： The content of astragaloside Ⅳ in Gansu Hedysari Radix was 0. 055 5 μg·g^-1,and the content of astragaloside Ⅳ in Astragali Radix was 37. 9 μg·g-1. The content of total flavonoids in two years old Hedysari Radix was as follows： 0. 449% in Longxi and other places,0. 370% in Dangchang,0. 394% in Wudu,and 0. 402% in the whole province; the content of total flavonoids in two years old Astragali Radix was as follows：0. 374% in Longxi and other places,0. 542% in Dangchang,0. 424% in Wudu, and 0. 452% in the whole province; the content of total saponins in two years old Hedysari Radix was as follows： 3. 68% in Longxi and other places,3. 89% in Dangchang,4. 24% in Wudu, and 3. 95% in the whole province; the content of total saponins in two years old Astragali Radix was as follows： 4. 63% in Longxi and other places,5. 11% in Dangchang,4. 80% in Wudu,and 4. 86% in the whole province. Conclusion： Gansu Hedysari Radix contained astragaloside A,but was only 1/682 of that in Astragali Radix. The contents of total flavonoids and total saponins in one year old and two years old Hedysari Radix were lower than those in Astragali Radix. Th

关 键 词：红芪 黄芪 总黄酮 总皂苷 紫外-可见分光光度法 含量对比 

分 类 号：R284.1[医药卫生—中药学] R282.4[医药卫生—中医学]