脂肪因子网膜素1与妊娠期糖尿病胰岛素抵抗的相关性研究  

Relationship of Omentin-1and insulin resistant in gestational diabetes mellitus

作　　者：李晓红[1] 陈卓[1] 马润玫[1] 田玉芹[1] 李红瑜[1] LI Xiaohong;CHEN Zhuo;MA Runmei(Department of Gynecology and Obstetrics, The First Affiliated Hospital of Kunming Medical College, Kunming 650031, China)

机构地区：[1]昆明医科大学第一附属医院妇产科,650031

出　　处：《中国糖尿病杂志》2018年第6期446-450,共5页Chinese Journal of Diabetes

基　　金：国家自然科学基金(81160082)

摘　　要：目的通过检测GDM患者血清及大网膜脂肪组织中网膜素1(Omentin-1)及胰岛素受体底物1(IRS-1)、胰岛素受体底物2(IRS-2)的表达,探讨脂肪因子Omentin-1与GDM患者IR的关系。方法将研究对象分为GDM组26例、正常对照组(GNGT)24名。产前ELISA测定空腹血清Omentin-1水平;剖宫产术中收集大网膜脂肪组织,RT-PCR及Western blot检测其Omentin-1、IRS-1、IRS-2mRNA及蛋白表达。结果 GDM组血清中Omentin-1较GNGT组低[(217.88±72.15)vs(272.08±107.30)ng/ml,P=0.048]。GDM组大网膜脂肪组织中IRS-1mRNA及蛋白表达较GNGT组降低(P<0.05),IRS-2在两组大网膜组织表达比较,差异无统计学意义;Omentin-1在GDM组大网膜脂肪组织中及蛋白表达降低,但差异无统计学意义。大网膜脂肪组织中Omentin-1 mRNA表达与IRS-1(r=0.755,P=0.000)及IRS-2mRNA(r=0.352,P=0.012)呈正相关。结论 Omentin-1表达降低与GDM患者IR有关。Objective To explore the relationship between Omentin-1 and insulin resistant in women with gestational diabetes mellitus（GDM）. Methods 26 GDM and 24 healthy control subjects were enrolled in this study.Serum Omentin-1 was measured in ELISA method.The expression of Omenitn-1 and IRS-1,IRS-2 in visceral Omental adipose tissue were detected by quantitative real-time RT-PCR and Western blot. Results The serum Omentin-1 level was significantly decreased in GDM group（P=0.048）.The expression of Omentin-1 mRNA was decreased in GDM group,but there was no statistical significance.The expression of IRS-1 mRNA（P=0.049）and protein（P=0.020）in omental tissue were significantly decreased in women with GDM.There were no differences in the expression levels of IRS-2 mRNA and protein between two groups.The expression levels of Omenitn-1 were positively associated with IRS-1（r=0.755,P=0.000）and IRS-2（r=0.352,P=0.012）in visceral omental adipose tissue.Conclusion Obesity-induced low expression of Omenitn-1 inhibits IRS-1,IRS-2 activation and impairs the glucose metabolism.

关 键 词：网膜素1 糖尿病 妊娠 胰岛素抵抗 

分 类 号：R714.256[医药卫生—妇产科学]