高效液相色谱—串联质谱多反应监测鉴定人精浆脂质成分  

Identification of lipid composition in human seminal plasma by HPLC-MS/MS multiple reaction monitoring

作　　者：曹祺 靳宝军 程洁 王晶晶 陈书强 王晓红 Cao Qi;Jin Bao-jun;Cheng Jie;Wang Jing-jing;Chen Shu-qiang;Wang Xiao-hong(Department of Gynecology and Obstetrics, Reproductive Medicine Center, Tangdu Hospital of Air Force Medical University, Xi＇an, 710038, China;Unit 93897 of People＇s Liberation Army, Xi＇an 710077, China)

机构地区：[1]空军军医大学唐都医院妇产科生殖医学中心 [2]中国人民解放军93897部队

出　　处：《兰州大学学报（医学版）》2018年第3期23-30,共8页Journal of Lanzhou University（Medical Sciences）

基　　金：陕西省科技攻关计划项目(2017ZDCXL-SF-02-03)

摘　　要：目的鉴定男性精浆中脂质成分,为其在生殖领域中的研究和应用提供参考。方法采用高效液相色谱—串联质谱(HPLC-MS/MS)多反应监测技术,对人精浆中脂质类化合物成分及含量进行分析。结果共鉴定出24个脂质大类,包含273种脂质种类。按化学结构可分为5类:甾醇类,包括游离胆固醇(Cho)、胆固醇酯(CE);甘油酯类,包括三酰甘油(TAG)、二酰甘油(DAG);磷脂酰胆碱类,包括卵磷脂(PC)、磷脂酰乙醇胺(PE)、磷脂酰甘油(PG)、磷脂酰肌醇(PI)、磷脂酰丝氨酸(PS)、磷脂酸(PA);溶血磷脂类,包括溶血磷脂二酸(LBPA)、溶血磷脂酰胆碱(LPC)、溶血磷脂酰乙醇胺(LPE)、溶血磷脂酸(LPA)、溶血磷脂酰肌醇(LPI)、溶血磷脂酰丝氨酸(LPS);鞘脂类,包括鞘磷脂(SM)、神经酰胺(Cer)、葡糖神经酰胺(Glu Cer)、乳糖神经酰胺(Lac Cer)、神经节苷脂(GM3)、红细胞糖鞘脂(Gb3)、1-磷酸鞘氨(S1P)、鞘氨醇(Sph)。在这24个脂质大类中,相对含量最高的前3类分别是Cho、SM和PC,包含种类最多的前3类分别是PC、PE和TAG。结论应用脂质组学分析方法显示男性精浆脂质,为今后分析不同病理状态下精浆内各种脂质代谢物的变化、了解脂质代谢的特征提供数据基础,为将来通过HPLC-MS/MS脂质组学技术发掘可预测精子质量的敏感、特异的脂质标志物提供新技术手段。Objective To identify the lipid composition in male seminal plasma, thus providing reference for the study and application of lipidomics in the field of male reproduction. Methods High performance liquid chromatography-tandem mass spectrometry（HPLC-MS/MS） was used to analyze the composition and content of lipids in human seminal plasma. Results A total of 24 lipid classes including 273 species were identified. According to the chemical structure, these 24 classes could be divided into 5 categories： sterols[including free cholesterols（Cho）, cholesterol ester（CE）]; glycerides [including triacylglycerols（TAG）, diacylglycerols（DAG）]; phosphatidylcholines [including phosphatidylcholines（PC）, phosphatidylethanolamines（PE）, phosphatidylglycerols（PG）, phosphatidylinositols（PI）, phophatidylserines（PS）, phosphatidic acids（PA）]; lysophospholipids [including lyso-bisphosphatidic acids（LBPA）, lyso-PC（LPC）, lyso-PE（LPE）,lyso-PA（LPA）, lyso-PI（LPI）, lyso-PS（LPS）] and sphingolipids [including sphingomyelins（SM）, ceramides（Cer）, glucosylceramides（Glu Cer）, lactosylceramides（Lac Cer）, monosialo-dihexosyl gangliosides（GM3）, Globotriaosylceramide（Gb3）, sphingosine-1-phosphates（S1 P）, sphingosines（Sph）]. Of these 24 lipid classes, the top 3 with a relative content were Cho, SM and PC; the top 3 with a species number were PC, PE and TAG. Conclusion Through systematic and sensitive analytical methods, we displayed lipidomics of male seminal plasma, thus providing a data basis for analyzing the changes of lipid metabolites of male seminal plasma in different pathological conditions and comprehending the characteristics of lipid metabolism and, meanwhile, providing new means for exploring lipid markers that can sensitively and specially predict the semen quality with the technology of HPLC-MS/MS.

关 键 词：精浆 脂质组学 高效液相色谱—串联质谱 多反应监测 

分 类 号：R816.7[医药卫生—放射医学]