基于黄酮醇的高选择性半胱氨酸荧光探针的合成及性能研究  

Synthesis and properties of a highly selective flavonol-based fluorescent probe for the detection of cysteine

作　　者：胡云[1] 侯慧静[1] 胡金梅 HU Yun;HOU Hui-jing;HU jin-mei(Department of Bioengineering, Zhuhai Campus, Zunyi Medical College, Zhuhai 519041)

机构地区：[1]遵义医学院珠海校区生物工程系,珠海519041

出　　处：《分析试验室》2018年第7期745-750,共6页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金项目(81560562);贵州省科技厅省科技合作计划项目(黔科合LH字[2015]7559号);贵州省教育厅自然科学研究青年项目(黔教合KY字[2014]302)资助

摘　　要：报道了一种基于4’-二乙氨基黄酮醇、以丙烯酸酯为半胱氨酸(Cys)反应基团的荧光增强型探针A1,对Cys的检测响应快速(5 min内),能有效识别区分另外两种含巯基生物分子高半胱氨酸(Hcy)和谷胱甘肽(GSH)。探针溶液荧光强度与加入的Cys浓度呈线性相关,拟合方程为y=6.894x+0.8409(R^2=0.9973),检测限为1×10^(-7)mol/L。加入Cys后,探针溶液由浅色变为亮黄色,在自然光条件下实现对Cys的比色检测。检测机理推测为Cys对A1中的丙烯酰基进行了共轭加成并使酯键断裂,使荧光母体得到释放从而产生增强的荧光信号。探针A1可用于活细胞内对Cys的荧光成像分析。Aturn-on fluorescent probe that can be used for rapid and specific detection of Cystine（ Cys） was reported in this work. This probe employed 4＇-diethylamino-3-hydroxyflavone as the fluorophore and an acrylate group as the recognition unit. Upon addition of Cys,the probe exhibited a color change from colorless to yellow under room light,and the fluorescent intensity（ λem= 537 nm）increased quickly and remarkably. A good linear relationship was shown between fluorescent intensity and concentrations of Cys. The linear equation was y = 6. 894 x ＋ 0. 8409（ R2= 0. 9973） and the detection limit was calculated as 1 × 10-7 mol/L. No apparent fluorescent response or color change was observed when exposing the probe to other amino acids or biothiols,such as Homocysteine（ Hcy） and Glutahione（ GSH）. Sensing mechanism investigation suggested that Cys could specically trigger the cleavage of acryloyl group and simultaneously release the fluorophore. Finally, bioimaging of intracellular Cys by this probe was successfully performed in living cells,which indicated that the probe had a great potential for biological applications.

关 键 词：荧光探针 半胱氨酸 黄酮醇 生物巯基 

分 类 号：O657.3[理学—分析化学]